Počet záznamů: 1
Analytical isotachophoresis of lactate in human serum using dry film photoresist microfluidic chips compatible with a commercially available field-deployable instrument platform
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SYSNO ASEP 0398860 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Analytical isotachophoresis of lactate in human serum using dry film photoresist microfluidic chips compatible with a commercially available field-deployable instrument platform Tvůrce(i) Smejkal, P. (AU)
Breadmore, M. C. (AU)
Guijt, R. M. (AU)
Foret, František (UIACH-O) RID, ORCID
Bek, F. (DE)
Macka, M. (AU)Celkový počet autorů 6 Zdroj.dok. Analytica Chimica Acta. - : Elsevier - ISSN 0003-2670
Roč. 803, NOV (2013), s. 135-142Poč.str. 8 s. Forma vydání Tištěná - P Jazyk dok. eng - angličtina Země vyd. NL - Nizozemsko Klíč. slova isotachophoresis ; microfluidics ; fluorescence ; indirect detection ; serum Vědní obor RIV CB - Analytická chemie, separace CEP GAP301/11/2055 GA ČR - Grantová agentura ČR Institucionální podpora UIACH-O - RVO:68081715 UT WOS 000326684000016 DOI 10.1016/j.aca.2013.01.046 Anotace A dry film resist (DFR) chip compatible with the Agilent Bioanalyzer 2100 was designed and fabricated for use in the analysis of lactate in serum by chip isotachophoresis (ITP). The Agilent Bioanalyzer 2100 is a commercially available field deployable analytical instrument originally developed for the electrophoretic analysis of DNA, RNA and proteins. The DFR chip was designed for the ITP separation of lactate in human serum within 1 min and was made compatible with the Bioanalyzer after packaging in the plastic caddies normally used for the DNA chips. A 20-fold improvement in sensitivity was obtained for the DFR chips in comparison with the standard chips used in earlier work. The limit of detection and limit of quantification for lactate were 24 M and 80 M, respectively. This new approach enables the use of commercial platforms like the Agilent Bioanalyzer for new applications including the analysis of small molecules. Pracoviště Ústav analytické chemie Kontakt Iveta Drobníková, drobnikova@iach.cz, Tel.: 532 290 234 Rok sběru 2014
Počet záznamů: 1