Počet záznamů: 1
Heterosubtypic protection against influenza A induced by adenylate cyclase toxoids delivering conserved HA2 subunit of hemagglutinin
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SYSNO ASEP 0396067 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Heterosubtypic protection against influenza A induced by adenylate cyclase toxoids delivering conserved HA2 subunit of hemagglutinin Tvůrce(i) Staneková, Z. (SK)
Adkins, Irena (MBU-M)
Kosová, Martina (MBU-M)
Janulíková, J. (SK)
Šebo, Peter (MBU-M) RID, ORCID
Varečková, E. (SK)Zdroj.dok. Antiviral Research. - : Elsevier - ISSN 0166-3542
Roč. 97, č. 1 (2013), s. 24-35Poč.str. 11 s. Jazyk dok. eng - angličtina Země vyd. NL - Nizozemsko Klíč. slova Bordetella adenylate cyclase toxoid ; Influenza A infection ; Cross-protection Vědní obor RIV FR - Farmakologie a lékárnická chemie CEP GA310/08/0447 GA ČR - Grantová agentura ČR GP310/09/P582 GA ČR - Grantová agentura ČR Institucionální podpora MBU-M - RVO:61388971 UT WOS 000314332000003 DOI 10.1016/j.antiviral.2012.09.008 Anotace The protective efficacy of currently available influenza vaccines is restricted to vaccine strains and their close antigenic variants. A new strategy to obtain cross-protection against influenza is based on conserved antigens of influenza A viruses (IAV), which are able to elicit a protective immune response. Here we describe a vaccination approach involving the conserved stem part of hemagglutinin, the HA2 subunit, shared by different HA subtypes of IAV. To increase its immunogenicity, a novel strategy of antigen delivery to antigen presenting cells (APCs) has been used. The HA2 segment (residues 23-185) was inserted into a genetically detoxified adenylate cyclase toxoid (CyaA-E5) which specifically targets and penetrates CD11b-expressing dendritic cells. The CyaA-E5-HA2 toxoid induced HA2(93-102), HA2(96-104) and HA2(170-178)-specific and Th1 polarized T-cell responses, and also elicited strong broadly cross-reactive HA2-specific antibody response. BALB/c mice immunized with three doses of purified CyaA-E5-HA2 without any adjuvant recovered from influenza infection 2 days earlier than the control mock-immunized mice Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2014
Počet záznamů: 1