Počet záznamů: 1
Administration of anti-CD25 mAb leads to impaired alpha-galactosylceramide-mediated induction of IFN-gamma production in a murine model
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SYSNO ASEP 0395907 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Administration of anti-CD25 mAb leads to impaired alpha-galactosylceramide-mediated induction of IFN-gamma production in a murine model Tvůrce(i) Rosalia, Rodney Alexander (UMG-J)
Štěpánek, Ivan (UMG-J) RID
Polláková, Veronika (UMG-J)
Šímová, Jana (UMG-J) RID
Bieblová, Jana (UMG-J)
Indrová, Marie (UMG-J) RID
Moravcová, Simona (UMG-J) RID
Přibylová, Hana (UMG-J)
Bontkes, H. (NL)
Bubeník, Jan (UMG-J)
Sparwasser, T. (DE)
Reiniš, Milan (UMG-J) RIDZdroj.dok. Immunobiology. - : Elsevier - ISSN 0171-2985
Roč. 218, č. 6 (2013), s. 851-859Poč.str. 9 s. Jazyk dok. eng - angličtina Země vyd. DE - Německo Klíč. slova cancer immunotherapy ; DEREG mice ; interferon γ ; natural killer T cells ; PC61 monoclonal antibody ; T regulatory cells Vědní obor RIV EB - Genetika a molekulární biologie CEP GA301/07/1410 GA ČR - Grantová agentura ČR GAP301/10/2174 GA ČR - Grantová agentura ČR Institucionální podpora UMG-J - RVO:68378050 CEZ AV0Z50520514 - UMG-J (2005-2011) UT WOS 000319486200003 DOI 10.1016/j.imbio.2012.10.012 Anotace CD4(+)CD25(+)Foxp3(+) T regulatory cells (Tregs) and CD1d-restricted invariant natural killer T (iNKT) cells are two cell types that are known to regulate immune reactions. Depletion or inactivation of Tregs using specific anti-CD25 antibodies in combination with immunostimulation is an attractive modality especially in anti-tumour immunotherapy. However, CD25 is not expressed exclusively on Tregs but also on subpopulations of activated lymphocytes. Therefore, the modulatory effects of the specific anti-CD25 antibodies can also be partially attributed to their interactions with the effector cells. Here, the effector functions of iNKT cells were analysed in combination with anti-CD25 mAb PC61. Upon PC61 administration, alpha-galactosylceramide (alpha-GalCer)-mediated activation of iNKT cells resulted in decreased IFN-gamma but not IL-4 production. In order to determine whether mutual interactions between Tregs and iNKT cells take place, we compared IFN gamma production after alpha-GalCer administration in anti-CD25-treated and "depletion of regulatory T cell" (DEREG) mice. Since no profound effects on IFN gamma induction were observed in DEREG mice, deficient in FoxP3(+) Tregs, our results indicate that the anti-CD25 antibody acts directly on CD25(+) effector cells. In vivo experiments demonstrated that although both alpha-GalCer and PC61 administration inhibited TC-1 tumour growth in mice, no additive/synergic effects were observed when these substances were used in combination therapy. (C) 2012 Elsevier GmbH. All rights reserved. Pracoviště Ústav molekulární genetiky Kontakt Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217 Rok sběru 2014
Počet záznamů: 1