Počet záznamů: 1
Exploring chip-capillary electrophoresis-laser-induced fluorescence field-deployable platform flexibility: Separations of fluorescent dyes by chip-based non-aqueous capillary electrophoresis
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SYSNO ASEP 0395838 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Exploring chip-capillary electrophoresis-laser-induced fluorescence field-deployable platform flexibility: Separations of fluorescent dyes by chip-based non-aqueous capillary electrophoresis Tvůrce(i) Nuchtavorn, N. (IE)
Smejkal, Petr (UIACH-O)
Breadmore, M. C. (AU)
Guijt, R. M. (AU)
Doble, P. (AU)
Bek, F. (DE)
Foret, František (UIACH-O) RID, ORCID
Suntornsuk, L. (TH)
Macka, M. (AU)Celkový počet autorů 9 Zdroj.dok. Journal of Chromatography A. - : Elsevier - ISSN 0021-9673
Roč. 1286, APR (2013), s. 216-221Poč.str. 6 s. Forma vydání Tištěná - P Jazyk dok. eng - angličtina Země vyd. NL - Nizozemsko Klíč. slova microfluidic chip CE ; capillary electrophoresis ; NACE ; LIF detection Vědní obor RIV CB - Analytická chemie, separace CEP GBP206/12/G014 GA ČR - Grantová agentura ČR Institucionální podpora UIACH-O - RVO:68081715 UT WOS 000317156100025 DOI 10.1016/j.chroma.2013.02.060 Anotace We studied the chip compatibility with organic solvents (i.e. MeOH, ACN, DMF and DMSO) and demonstrated the chip compatibility with DMSO as a non-volatile and non-hazardous solvent with satisfactory stability of migration times over 50 h. The generic CE capability is illustrated with separations of fluorescent basic blue dyes methylene blue (MB), toluidine blue (TB), nile blue (NB) and brilliant cresyl blue (BC). Further, the effects of the composition of the background electrolyte (BGE) on the separation were studied, including the contents of water (0-30%) and buffer composition. In background electrolytes containing typically 80 mmol/L ammonium acetate and 870 mmol/L acetic acid in 100% DMSO baseline separation of the dyes were achieved in 40 s. Linearity was documented in the range of 5-28 mu mol/L, 10-100 mu mol/L, 1.56-50 nmol/L and 5-75 nmol/L (r(2) values in the range 0.974-0.999), and limit of detection (LOD) values were 90 nmol/L, 1 mu mol/L 1.4 nmol/L, and 2 nmol/L for MB, TB, NB and BC, respectively. (c) 2013 Elsevier B.V. All rights reserved. Pracoviště Ústav analytické chemie Kontakt Iveta Drobníková, drobnikova@iach.cz, Tel.: 532 290 234 Rok sběru 2014
Počet záznamů: 1