Počet záznamů: 1
Nucleotide Excision Repair Is Not Induced in Human Embryonic Lung Fibroblasts Treated with Environmental Pollutants
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SYSNO ASEP 0395392 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Nucleotide Excision Repair Is Not Induced in Human Embryonic Lung Fibroblasts Treated with Environmental Pollutants Tvůrce(i) Rössner ml., Pavel (UEM-P) RID, ORCID
Mrhálková, Andrea (UEM-P)
Uhlířová, Kateřina (UEM-P)
Špátová, Milada (UEM-P)
Rössnerová, Andrea (UEM-P) RID
Líbalová, Helena (UEM-P) RID
Schmuczerová, Jana (UEM-P) RID
Milcová, Alena (UEM-P)
Topinka, Jan (UEM-P) RID, ORCID
Šrám, Radim (UEM-P) RIDCelkový počet autorů 10 Zdroj.dok. PLoS ONE. - : Public Library of Science - ISSN 1932-6203
Roč. 8, č. 7 (2013), e69197Poč.str. 12 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova environmental pollutants ; DNA ; expression of DNA Vědní obor RIV DN - Vliv životního prostředí na zdraví CEP GAP503/11/0084 GA ČR - Grantová agentura ČR Institucionální podpora UEM-P - RVO:68378041 UT WOS 000322391400043 EID SCOPUS 84880403244 DOI 10.1371/journal.pone.0069197 Anotace We tested response of normal human embryonic lung fibroblasts (HEL12469 cells) to treatment with benzo[a]pyrene (B[a]P) and extractable organic matter (EOM) from ambient air particles (PM2.5) collected in two Czech cities differing in levels and sources of air pollution. We analyzed multiple endpoints associated with exposure to PAHs: bulky DNA adducts and the nucleotide excision repair (NER) response [expression of XPE, XPC and XPA genes on the level of mRNA and proteins, unscheduled DNA synthesis (UDS)]. EOMs were collected in the winter and summer of 2011 in two Czech cities with different levels and sources of air pollution. The levels of bulky DNA adducts were highest after treatment with B[a]P, followed by winter EOMs; their induction by summer EOMs was weak. The induction of both mRNA and protein expression was observed, with the most pronounced effects after treatment with B[a]P (–S9); the response induced by EOMs from both cities and seasons was substantially weaker. The expression of DNA repair genes was not accompanied by the induction of UDS activity. Our results indicate that the tested compounds induced low levels of DNA damage and affected the expression of NER genes; however, nucleotide excision repair was not induced. Pracoviště Ústav experimentální medicíny Kontakt Lenka Koželská, lenka.kozelska@iem.cas.cz, Tel.: 241 062 218, 296 442 218 Rok sběru 2014
Počet záznamů: 1