Počet záznamů: 1

The tracing of mycobacteria in drinking water supply systems by culture, conventional, and real time PCRs

SYSNO ASEP	0392327
Druh ASEP	J - Článek v odborném periodiku
Zařazení RIV	J - Článek v odborném periodiku
Poddruh J	Článek ve WOS
Název	The tracing of mycobacteria in drinking water supply systems by culture, conventional, and real time PCRs
Tvůrce(i)	Klanicová, B. (CZ) Seďa, Jaromír (BC-A)_RID Slaná, I. (CZ) Slaný, M. (CZ) Pavlík, I. (CZ)
Zdroj.dok.	Current Microbiology. - : Springer - ISSN 0343-8651 Roč. 67, č. 6 (2013), s. 725-731
Poč.str.	7 s.
Jazyk dok.	eng - angličtina
Země vyd.	US - Spojené státy americké
Klíč. slova	avium subsp paratuberculosis ; nontuberculous mycobacteria ; potable water ; survival ; intracellulare
Vědní obor RIV	EE - Mikrobiologie, virologie
Institucionální podpora	BC-A - RVO:60077344
UT WOS	000325813400015
EID SCOPUS	84886600295
DOI	10.1007/s00284-013-0427-1
Anotace	Mycobacteria are widely present in diverse aquatic habitats, where they can survive for months or years while some species can even proliferate. The resistance of different mycobacterial species to disinfection methods like chlorination or ozonation could result in their presence in the final tap water of consumers. In this study, the culture method, Mycobacterium tuberculosis complex conventional duplex PCR for detection of non-tuberculous mycobacteria (NTM) and quantitative real-time PCR (qPCR) to detect three subspecies of M. avium species (M. a. avium, M. a. hominissuis, and M. a. paratuberculosis) were used to trace their possible path of transmission from the watershed through the reservoir and drinking water plant to raw drinking water and finally to households. A total of 124 samples from four drinking water supply systems in the Czech Republic, 52 dam sediments, 34 water treatment plant sludge samples, and 38 tap water household sediments, were analyzed. NTM of 11 different species were isolated by culture from 42 (33.9 %) samples; the most prevalent were M. gordonae (16.7 %), M. triplex (14.3 %), M. lentiflavum (9.5 %), M. a. avium (7.1 %), M. montefiorenase (7.1 %), and M. nonchromogenicum (7.1 %). NTM DNA was detected in 92 (76.7 %) samples. By qPCR analysis a statistically significant decrease (P < 0.01) was observed along the route from the reservoir (dam sediments), through water treatment sludge and finally to household sediments. The concentrations ranged from 10(0) to 10(4) DNA cells/g. It was confirmed that drinking water supply systems (watershed-reservoir-drinking water treatment plant-household) might be a potential transmission route for mycobacteria.
Pracoviště	Biologické centrum (od r. 2006)
Kontakt	Dana Hypšová, eje@eje.cz, Tel.: 387 775 214
Rok sběru	2014

Počet záznamů: 1