Počet záznamů: 1

De Novo Biosynthesis of Sexual Pheromone in the Labial Gland of Bumblebee Males

SYSNO ASEP	0391726
Druh ASEP	J - Článek v odborném periodiku
Zařazení RIV	J - Článek v odborném periodiku
Poddruh J	Článek ve WOS
Název	De Novo Biosynthesis of Sexual Pheromone in the Labial Gland of Bumblebee Males
Tvůrce(i)	Žáček, Petr (UOCHB-X)_RID Prchalová-Horňáková, Darina (UOCHB-X) Tykva, Richard (UOCHB-X) Kindl, Jiří (UOCHB-X)_RID Vogel, H. (DE) Svatoš, Aleš (UOCHB-X)_{ORCID, RID} Pichová, Iva (UOCHB-X)_{RID, ORCID} Valterová, Irena (UOCHB-X)_{RID, ORCID}
Celkový počet autorů	8
Zdroj.dok.	Chembiochem. - : Wiley - ISSN 1439-4227 Roč. 14, č. 3 (2013), s. 361-371
Poč.str.	11 s.
Jazyk dok.	eng - angličtina
Země vyd.	DE - Německo
Klíč. slova	labial gland ; Bombus lucorum ; Bombus terrestris ; fat bodies ; gene expression
Vědní obor RIV	CE - Biochemie
CEP	GA203/09/1446 GA ČR - Grantová agentura ČR
Institucionální podpora	UOCHB-X - RVO:61388963
UT WOS	000315491700012
EID SCOPUS	84873489090
DOI	10.1002/cbic.201200684
Anotace	De novo biosynthesis of male sex pheromone from two bumblebee species (Bombus terrestris and Bombus lucorum) was studied by using in vitro incubations of labial glands (LGs) with radioactive [1,2-C-14] acetate and deuterated [D-3]acetate. The labeled substrate was incorporated into several types of compounds, such as terpenic alcohols, fatty acids, esters, and hydrocarbons. A similar incubation of [1,2-C-14] acetate with fat bodies (FB) led to the formation of fatty acids, triacylglycerols (TAG), and hydrocarbons. To support the results from in vitro incubations, PCR analysis of fatty acid synthase (FAS) transcripts in LG and FB was performed. Relative quantification of FAS transcription levels revealed that the abundance of mRNA from the FAS gene is a function of the age of B. terrestris males. A comparison of the relative FAS mRNA gene transcription level in FB and LGs of B. terrestris and B. lucorum males proved that high biosynthetic activity takes place in the LGs of both species. Together, these results indicate that pheromone components are synthesized de novo in the LG.
Pracoviště	Ústav organické chemie a biochemie
Kontakt	asep@uochb.cas.cz ; Kateřina Šperková, Tel.: 232 002 584 ; Viktorie Chládková, Tel.: 232 002 434
Rok sběru	2014

Počet záznamů: 1