Počet záznamů: 1  

Engineered drought tolerance in tomato plants is reflected in chlorophyll fluorescence emission

  1. 1.
    SYSNO ASEP0388804
    Druh ASEPJ - Článek v odborném periodiku
    Zařazení RIVJ - Článek v odborném periodiku
    Poddruh JČlánek ve WOS
    NázevEngineered drought tolerance in tomato plants is reflected in chlorophyll fluorescence emission
    Tvůrce(i) Mishra, Kumud (UEK-B) RID, ORCID, SAI
    Iannacone, R. (IT)
    Petrozza, A. (IT)
    Mishra, Anamika (UEK-B) RID
    Armentano, N. (IT)
    La Vecchia, G. (IT)
    Trtílek, M. (CZ)
    Cellini, F. (IT)
    Nedbal, Ladislav (UEK-B)
    Zdroj.dok.Plant Science. - : Elsevier - ISSN 0168-9452
    Roč. 182, SI (2012), s. 79-86
    Poč.str.8 s.
    Jazyk dok.eng - angličtina
    Země vyd.IE - Irsko
    Klíč. slovaChlorophyll fluorescence ; Drought ; High-throughput screening ; Solanum lycopersicum ; Transcription factor ; Transgenic plant
    Vědní obor RIVEH - Ekologie - společenstva
    CEPOC08055 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy
    2B06068 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy
    ED1.1.00/02.0073 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy
    Institucionální podporaRVO:67179843 - RVO:67179843
    UT WOS000298723200010
    DOI10.1016/j.plantsci.2011.03.022
    AnotaceDrought stress is one of the most important factors that limit crop productivity worldwide. In order to obtain tomato plants with enhanced drought tolerance, we inserted the transcription factor gene ATHB-7 into the tomato genome. This gene was demonstrated earlier to be up-regulated during drought stress in Arabidopsis thaliana thus acting as a negative regulator of growth. We compared the performance of wild type and transgenic tomato line DTL-20, carrying ATHB-7 gene, under well-irrigated and water limited conditions. We found that transgenic plants had reduced stomatal density and stomatal pore size and exhibited an enhanced resistance to soil water deficit. We used the transgenic plants to investigate the potential of chlorophyll fluorescence to report drought tolerance in a simulated high-throughput screening procedure. Wild type and transgenic tomato plants were exposed to drought stress lasting 18 days. The stress was then terminated by rehydration after which recovery was studied for another 2 days. Plant growth, leaf water potential, and chlorophyll fluorescence were measured during the entire experimental period. We found that water potential in wild type and drought tolerant transgenic plants diverged around day 11 of induced drought stress. The chlorophyll fluorescence parameters: the non-photochemical quenching, effective quantum efficiency of PSII, and the maximum quantum yield of PSII photochemistry yielded a good contrast between wild type and transgenic plants from day 7, day 12, and day 14 of induced stress, respectively. We propose that chlorophyll fluorescence emission reports well on the level of water stress and, thus, can be used to identify elevated drought tolerance in high-throughput screens for selection of resistant genotypes.
    PracovištěÚstav výzkumu globální změny
    KontaktNikola Šviková, svikova.n@czechglobe.cz, Tel.: 511 192 268
    Rok sběru2013
Počet záznamů: 1  

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