Počet záznamů: 1
Impact of oxidized phospholipids on the structural and dynamic organization of phospholipid membranes: a combined DSC and solid state NMR study
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SYSNO ASEP 0388697 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Impact of oxidized phospholipids on the structural and dynamic organization of phospholipid membranes: a combined DSC and solid state NMR study Tvůrce(i) Wallgren, M. (SE)
Beranová, Lenka (UFCH-W)
Pham, Q. D. (SE)
Linh, K. (SE)
Lidman, M. (SE)
Procek, J. (PL)
Cyprych, K. (PL)
Kinnunen, P. K. J. (FI)
Hof, Martin (UFCH-W) RID, ORCID
Gröbner, G. (SE)Zdroj.dok. Faraday Discussions. - : Royal Society of Chemistry - ISSN 1359-6640
Roč. 161, DEC 2013 (2013), s. 499-513Poč.str. 15 s. Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova ACYL-CHAIN REVERSAL ; MOLECULAR SIMULATIONS ; LIPID-MEMBRANES Vědní obor RIV CF - Fyzikální chemie a teoretická chemie CEP GEMEM/09/E006 GA AV ČR - Akademie věd Institucionální podpora UFCH-W - RVO:61388955 UT WOS 000313970200025 EID SCOPUS 84883004226 DOI 10.1039/c2fd20089a Anotace Membranes undergo severe changes under oxidative stress conditions due to the creation of oxidized phospholipid (OxPL) species, which possess molecular properties quite different from their parental lipid components. These OxPLs play crucial roles in various pathological disorders and their occurrence is involved in the onset of intrinsic apoptosis, a fundamental pathway in programmed mammalian cell death. However, the molecular mechanisms by which these lipids can exert their apoptotic action via their host membranes (e.g., altering membrane protein function) are poorly understood. Therefore, we studied the impact of OxPLs on the organization and biophysical properties of 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) based lipid membranes by differential scanning calorimetry (DSC) and solid state nuclear magnetic resonance (NMR) spectroscopy. Incorporation of defined OxPLs with either a carboxyl group (1-palmitoyl-2-azelaoyl-sn-glycero-3-phosphocholine (PazePC)) or aldehyde (1-palmitoyl-(90oxononanoyl)-sn-glycero-3-phosphocholine (PoxnoPC)) at their truncated sn-2-chain ends enabled us to reveal OxPL speciesdependent differences. The calorimetric studies revealed significant effects of OxPLs on the thermotropic phase behavior of DMPC bilayers, especially at elevated levels where PazePC induced more pronounced effects than PoxnoPC. Pracoviště Ústav fyzikální chemie J.Heyrovského Kontakt Michaela Knapová, michaela.knapova@jh-inst.cas.cz, Tel.: 266 053 196 Rok sběru 2014
Počet záznamů: 1