Počet záznamů: 1
Production of high-density Chlorella culture grown in fermenters
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SYSNO ASEP 0388567 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Production of high-density Chlorella culture grown in fermenters Tvůrce(i) Doucha, Jiří (MBU-M)
Lívanský, Karel (MBU-M)Zdroj.dok. Journal of Applied Phycology. - : Springer - ISSN 0921-8971
Roč. 24, č. 1 (2012), s. 35-43Poč.str. 9 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova Chlorella vulgaris ; Heterotrophic culture ; Fed-batch Vědní obor RIV EE - Mikrobiologie, virologie CEP OE09025 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Institucionální podpora MBU-M - RVO:61388971 UT WOS 000299122200004 DOI 10.1007/s10811-010-9643-2 Anotace The freshwater microalga Chlorella vulgaris was grown heterotrophically in fed-batch 50-600-L fermenters at 36A degrees C, on aerated and mixed nutrient solution with urea as a nitrogen and glucose as a carbon and energy source. Cell density increased from the initial value 6.25 to 117.18 g DW L-1 in 32 h in the fermenter 50 L at a mean growth rate 3.52 g DW L-1 h(-1). The DW increase in the fermenter 200 L was from 7.25 to 94.82 g DW L-1 in 26.5 h at a mean growth rate 3.37 g DW L-1 h(-1). Mean specific growth rate mu was about 0.1 h(-1) in the both fermenters, if nutrients and oxygen were adequately supplied. The DW increase in the fermenter 600 L was from 0.8 to 81.6 g DW L-1 in 66.5 h at a mean growth rate 1.22 g DW L-1 h(-1) and mu = 0.07 h(-1). A limitation of the cell growth rate in 600 L fermenter caused by a low dissolved oxygen concentration above cell densities higher than 10 g DW L-1) occurred. Specific growth rate decreased approximately linearly with increasing glucose concentration (25-80 g glucose L-1) at the beginning of cultivation and decreased with the time of cultivation. The cell yield was 0.55-0.69 g DW (g glucose)(-1). The content of proteins, beta-carotene, and chlorophylls in the cells steadily increased and starch content decreased, by keeping aerated and mixed culture another 12 h in fermenter after the cell growth was stopped due to glucose deficiency Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2013
Počet záznamů: 1