Počet záznamů: 1
PIXE maps of intracellular element distribution in cerebellar neurons
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SYSNO ASEP 0388232 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Ostatní články Název PIXE maps of intracellular element distribution in cerebellar neurons Tvůrce(i) Kranda, Karel (UJF-V)
Havránek, Vladimír (UJF-V) RID, SAI, ORCID
Purkrtová, Z. (CZ)
Vožeh, F. (CZ)
Hájková, L. (CZ)Celkový počet autorů 5 Zdroj.dok. International journal of PIXE - ISSN 0129-0835
Roč. 22, 1-2 (2012), s. 65-72Poč.str. 8 s. Jazyk dok. eng - angličtina Země vyd. SG - Singapur Klíč. slova MicroPIXE ; 2D-mapping ; cerebellum ; mutant mice ; Purkinje cells ; cell death ; metan concentration Vědní obor RIV BG - Jaderná, atomová a mol. fyzika, urychlovače CEP GA309/09/1189 GA ČR - Grantová agentura ČR Institucionální podpora UJF-V - RVO:61389005 DOI 10.1142/S0129083512400190 Anotace Neurodegenerative disorders are accompanied by altered compositions of metals that may convey some information about the health and possibly metabolic state of neurons. Here we opted for PIXE technique to perform a spatially-resolved elemental analysis of intracellular space of individual neurons. Primarily, we measured element distribution within Purkinje cells (PC) of normal and Lurcher (Lc/+) mutant mice to detect any anomalies in concentrations of individual elements attributable to cell death. Lc/+ mutant mice provide an excellent animal model for time-course of excitotoxic cell death. PCs, some with autofluorescence, were scanned with a proton beam to record their X-ray emission and RBS spectra and to construct 2D-concentration maps of individual elements. PCs with auto-fluorescence, presumed to be either already dead or moribund, showed elevated concentrations of Cu, Fe, and Zn, when compared to healthy PCs. In conclusion, excitotoxic mode of death, as observed in the moribund PCs of Lc/+ cerebellum, may activate enzymatic processes, manifested as increased intracellular concentrations of Cu and Zn that are possibly constituents of some metalloenzyme complexes. Pracoviště Ústav jaderné fyziky Kontakt Markéta Sommerová, sommerova@ujf.cas.cz, Tel.: 266 173 228 Rok sběru 2013
Počet záznamů: 1