Počet záznamů: 1  

Identification of Bifidobacterium strains from faeces of lambs

  1. 1.
    SYSNO ASEP0387185
    Druh ASEPJ - Článek v odborném periodiku
    Zařazení RIVJ - Článek v odborném periodiku
    Poddruh JČlánek ve WOS
    NázevIdentification of Bifidobacterium strains from faeces of lambs
    Tvůrce(i) Bunešová, V. (CZ)
    Vlková, E. (CZ)
    Killer, Jiří (UZFG-Y) RID
    Rada, V. (CZ)
    Ročková, Š. (CZ)
    Zdroj.dok.Small Ruminant Research. - : Elsevier - ISSN 0921-4488
    Roč. 105, 1-3 (2012), 355-360
    Poč.str.6 s.
    Jazyk dok.eng - angličtina
    Země vyd.NL - Nizozemsko
    Klíč. slovabifidobacterium ; identification ; lambs
    Vědní obor RIVGH - Výživa hospodářských zvířat
    CEPGA523/08/1091 GA ČR - Grantová agentura ČR
    GD525/08/H060 GA ČR - Grantová agentura ČR
    Institucionální podporaUZFG-Y - RVO:67985904
    UT WOS000305100900058
    DOI10.1016/j.smallrumres.2011.12.004
    AnotaceBifidobacteria were enumerated and isolated from the faeces of lambs aged between 25 and 40 days using modified trypticase-phytone-yeast extract (TPY) agar supplemented with mupirocin and acetic acid. Nineteen strains were characterised and divided into 6 groups according their fermentation characteristics obtained by API 50 CHL and RAPID ID 32 tests and by randomly amplified polymorphic DNA (RAPD) profiles. One representative strain of each group was identified by sequencing by 16S rRNA and hsp60 genes sequencing as B. animalis ssp. animalis, B. choerinum, B. pseudolongum ssp. globosum, B. pseudocatenulatum. Examination of the DNA fingerprinting showed a genetic diversity between strains of B. animalis ssp. animalis and B. choerinum. Our result shows that some bifidobacterial species should not be host-specific or have a broad host spectrum. As bifidobacteria were determined as numerous bacterial group in the intestine of lambs on a milk diet (8.32 +/- 0.71 log CFU/g, mean count +/- S.D., n = 30), there is a reasonable presumption that bifidobacteria could be an effective probiotics for lambs. Identification techniques used in this study were found to be suitable for the classification of lamb origin bifidobacteria.
    PracovištěÚstav živočišné fyziologie a genetiky
    KontaktJana Zásmětová, knihovna@iapg.cas.cz, Tel.: 315 639 554
    Rok sběru2013