Počet záznamů: 1  

Application of UV-Vis spectrophotometric and chemiluminescent methods for the evaluation of the antioxidant action of curcumin

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    SYSNO ASEP0383640
    Druh ASEPJ - Článek v odborném periodiku
    Zařazení RIVJ - Článek v odborném periodiku
    Poddruh JČlánek ve WOS
    NázevApplication of UV-Vis spectrophotometric and chemiluminescent methods for the evaluation of the antioxidant action of curcumin
    Tvůrce(i) Stanchev, Stancho (UOCHB-X) RID
    Pencheva, I. (BG)
    Konstantinov, S. (BG)
    Obreshkova, D. (BG)
    Hadjimitova, V. (BG)
    Celkový počet autorů5
    Zdroj.dok.Journal of the Serbian Chemical Society - ISSN 0352-5139
    Roč. 77, č. 8 (2012), s. 1063-1069
    Poč.str.7 s.
    Jazyk dok.eng - angličtina
    Země vyd.RS - Srbsko
    Klíč. slovacurcumin ; antioxidant ; UV-Vis spectrophotometry ; DNA complexation ; chemiluminescence
    Vědní obor RIVCC - Organická chemie
    CEZAV0Z40550506 - UOCHB-X (2005-2011)
    UT WOS000308830900008
    DOI10.2298/JSC110621032S
    AnotaceCurcumin (1,7-bis(4-hydroxy-3-methoxyphenyl)-1,6-heptadiene-3,5-dione) is a natural biologically active substance with antioxidant activity. The ability of curcumin to inhibit free radical reactions can be used in the prevention of diseases such as cancer and coronary heart disease. UV-Vis spectrophotometric and chemiluminescent dynamic methods for determination of the antioxidant activity of curcumin were developed. The spectrophotometric method includes investigation of the interaction between DNA, isolated from HL-60 cells, and curcumin. The decreasing absorption of curcumin in the presence of HL-60 DNA against the blank sample can be a measurement for some complex formation between curcumin and DNA. The chemiluminescent method involves three tests for the detection of luminol- dependent chemiluminescence based on model systems that generate superoxide, hydroxide and hypochlorite radicals. The strongest decay of chemilunimescence was registered at the highest concentration of curcumin (100 mu mol L-1).
    PracovištěÚstav organické chemie a biochemie
    KontaktJana Procházková, janap@uochb.cas.cz, Tel.: 220 183 418 ; Viktorie Chládková, viktorie.chladkova@uochb.cas.cz, Tel.: 232 002 434
    Rok sběru2013
Počet záznamů: 1