Počet záznamů: 1
Electrophoretic and chromatographic evaluation of transgenic barley expressing a bacterial dihydrodipicolinate synthase
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SYSNO ASEP 0382218 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Electrophoretic and chromatographic evaluation of transgenic barley expressing a bacterial dihydrodipicolinate synthase Tvůrce(i) Ohnoutková, Ludmila (UEB-Q) RID, ORCID
Zítka, O. (CZ)
Mřížová, K. (CZ)
Vašková, Jana (UEB-Q) RID
Galuszka, P. (CZ)
Cernei, N. (CZ)
Smedley, M. A. (GB)
Harwood, W. A. (GB)
Adam, V. (CZ)
Křížek, R. (CZ)Zdroj.dok. Electrophoresis. - : Wiley - ISSN 0173-0835
Roč. 33, č. 15 (2012), s. 2365-2373Poč.str. 9 s. Jazyk dok. eng - angličtina Země vyd. DE - Německo Klíč. slova Foodomics ; Gel electrophoresis ; Polymerase chain reaction Vědní obor RIV EB - Genetika a molekulární biologie CEP 1M06030 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy CEZ AV0Z50380511 - UEB-Q (2005-2011) UT WOS 000307399400014 DOI 10.1002/elps.201200033 Anotace Nutritional quality of human and animal foodstuffs is determined by the content of essential amino acids. Barley is the fourth most important cereal of the world and the second most important cereal grown in the Czech Republic. Cereal grains such as barley contain insufficient levels of some essential amino acids, especially lysine. Dihydrodipicolinate synthase is the key enzyme involved in the regulatory step for lysine biosynthesis. Two constructs pBract214::sTPdapA and pBract214::mdapA containing the dapA gene from Escherichia coli coding for the bacterial dihydrodipicolinate synthase were used for transformation of barley. An Agrobacterium-mediated technique was used for transformation of immature embryos of spring barley cv. Golden Promise. Transgenic barley plants of the T0 and T1 generations were evaluated by PCR, real-time PCR, gel electrophoresis, and Western blot. Amino acid content was analyzed by HPLC after HCl hydrolysis. The lysine content in leaves of the T1 generation plant no. 5/5 was 50% higher than in wild-type plants; the lysine content in seeds of T2 generation plant no. 5/16 was 30% higher than in wild-type seeds of spring barley cv. Golden Promise. Pracoviště Ústav experimentální botaniky Kontakt David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Rok sběru 2013
Počet záznamů: 1