Počet záznamů: 1
Biophysical properties and cellular toxicity of covalent crosslinked oligomers of α-synuclein formed by photoinduced side-chain tyrosyl radicals
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SYSNO ASEP 0381273 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Biophysical properties and cellular toxicity of covalent crosslinked oligomers of α-synuclein formed by photoinduced side-chain tyrosyl radicals Tvůrce(i) Borsarelli, C.D. (AR)
Falomir-Lockhart, L.J. (DE)
Ostatná, Veronika (BFU-R) RID, ORCID
Fauerbach, J.A. (AR)
Hsiao, H.-H. (DE)
Urlaub, H. (DE)
Paleček, Emil (BFU-R) RID, ORCID
Jares-Erijman, E.A. (AR)
Jovin, T.M. (DE)Celkový počet autorů 9 Zdroj.dok. Free Radical Biology and Medicine. - : Elsevier - ISSN 0891-5849
Roč. 53, č. 4 (2012), s. 1004-1015Poč.str. 12 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova Parkinson's disease ; neurodegeneration ; oxidative stress Vědní obor RIV BO - Biofyzika CEP KJB100040901 GA AV ČR - Akademie věd CEZ AV0Z50040702 - BFU-R (2007-2013) UT WOS 000307920100035 DOI 10.1016/j.freeradbiomed.2012.06.035 Anotace In this work, we describe the preparation and characterization of low molecular weight covalently bound oligomeric species of alpha-synuclein (aS) obtained by crosslinking via tyrosyl radicals. Numerous analytical techniques were used to characterize the aS oligomers. The diverse oligomeric species exhibited a direct effect on the in vitro aggregation behavior of wild-type monomeric aS.Compared to the unmodified monomer, the photoinduced covalent oligomeric species demonstrated increased toxic effects on differentiated neuronal-like SH-SY5Y cells. The results highlight the importance of protein modification induced by oxidative stress in the initial molecular events leading to Parkinson’s disease. Pracoviště Biofyzikální ústav Kontakt Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244 Rok sběru 2013
Počet záznamů: 1