Počet záznamů: 1
Interactions of an Arabidopsis RanBPM homologue with LisH-CTLH domain proteins revealed high conservation of CTLH complexes in eukaryotes
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SYSNO ASEP 0380982 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Interactions of an Arabidopsis RanBPM homologue with LisH-CTLH domain proteins revealed high conservation of CTLH complexes in eukaryotes Tvůrce(i) Tomaštíková, Eva (UEB-Q) RID, ORCID
Cenklová, Věra (UEB-Q)
Kohoutová, Lucie (MBU-M) RID
Petrovská, Beáta (UEB-Q) RID, ORCID
Váchová, Lenka (UEB-Q)
Halada, Petr (MBU-M) RID, ORCID
Kočárová, Gabriela (MBU-M)
Binarová, Pavla (MBU-M) RID, ORCIDZdroj.dok. BMC Plant Biology. - : BioMed Central - ISSN 1471-2229
Roč. 12, č. 83 (2012)Poč.str. 14 s. Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova Arabidopsis homologue of RanBPM ; CTLH-complex ; LisH-CTLH domain proteins Vědní obor RIV EB - Genetika a molekulární biologie CEP GA204/07/1169 GA ČR - Grantová agentura ČR GP204/09/P155 GA ČR - Grantová agentura ČR GAP501/12/2333 GA ČR - Grantová agentura ČR LC06034 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy LC545 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy IAA500200719 GA AV ČR - Akademie věd CEZ AV0Z50380511 - UEB-Q (2005-2011) AV0Z50200510 - MBU-M (2005-2011) UT WOS 000307098700001 DOI 10.1186/1471-2229-12-83 Anotace Based on sequence similarity, we identified a homologue of the human RanBPM in Arabidopsis thaliana. AtRanBPM protein has highly conserved SPRY, LisH, CTLH and CRA domains. Cell fractionation showed that endogenous AtRanBPM or expressed GFP-AtRanBPM are mainly cytoplasmic proteins with only a minor portion detectable in microsomal fractions. AtRanBPM was identified predominantly in the form of soluble cytoplasmic complexes similar to 230 - 500 kDa in size. Immunopurification of AtRanBPM followed by mass spectrometric analysis identified proteins containing LisH and CRA domains; LisH, CRA, RING-U-box domains and a transducin/WD40 repeats in a complex with AtRanBPM. Homologues of identified proteins are known to be components of the C-terminal to the LisH motif (CTLH) complexes in humans and budding yeast. Microscopic analysis of GFP-AtRanBPM in vivo and immunofluorescence localization of endogenous AtRanBPM protein in cultured cells and seedlings of Arabidopsis showed mainly cytoplasmic and nuclear localization. Absence of colocalization with.-tubulin was consistent with the biochemical data and suggests another than a centrosomal role of the AtRanBPM protein. Conclusion: We showed that as yet uncharacterized Arabidopsis RanBPM protein physically interacts with LisH-CTLH domain-containing proteins. The newly identified high molecular weight cytoplasmic protein complexes of AtRanBPM showed homology with CTLH types of complexes described in mammals and budding yeast. Although the exact functions of the CTLH complexes in scaffolding of protein degradation, in protein interactions and in signalling from the periphery to the cell centre are not yet fully understood, structural conservation of the complexes across eukaryotes suggests their important biological role. Pracoviště Ústav experimentální botaniky Kontakt David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Rok sběru 2013
Počet záznamů: 1