Počet záznamů: 1

Characteristics of bovine oocytes with different meiotic competence in terms of their mitochondrial status and expression of nuclear-encoded factors

SYSNO ASEP	0380415
Druh ASEP	J - Článek v odborném periodiku
Zařazení RIV	J - Článek v odborném periodiku
Poddruh J	Článek ve WOS
Název	Characteristics of bovine oocytes with different meiotic competence in terms of their mitochondrial status and expression of nuclear-encoded factors
Tvůrce(i)	Machatková, M. (CZ) Jeseta, M. (CZ) Hulínská, P. (CZ) Knitlová, D. (CZ) Němcová, Lucie (UZFG-Y)_RID Kaňka, Jiří (UZFG-Y)_{RID, ORCID}
Celkový počet autorů	6
Zdroj.dok.	Reproduction in Domestical Animals. - : Wiley - ISSN 0936-6768 Roč. 47, č. 5 (2012), s. 806-814
Poč.str.	9 s.
Jazyk dok.	eng - angličtina
Země vyd.	DE - Německo
Klíč. slova	bovine oocytes ; mitochondrial status ; nuclear-encoded factors
Vědní obor RIV	GI - Šlechtění a plemenářství hospodářských zvířat
CEP	QI91A018 GA MZe - Ministerstvo zemědělství
CEZ	AV0Z50450515 - UZFG-Y (2005-2011)
UT WOS	000308087900020
DOI	10.1111/j.1439-0531.2011.01972.x
Anotace	The present study was designed to characterize bovine oocytes with different meiotic competence and atresia levels in terms of their mitochondrial status. Oocyte subpopulations were recovered either from medium (MF) or small (SF) follicles and categorized as healthy, light-atretic and mid-atretic according to oocyte morphology. Mitochondrial activity, morphology and distribution, adenosine triphosphate (ATP) content and expression of mitochondrial transcription factor A (TFAM) and nuclear respiratory factor 1 (NRF1) were assessed before (GV) and after (MII) maturation. The data were related to follicular size regardless of or with regard to oocyte atresia. Regardless of atresia, the MF subpopulation showed a significantly higher mitochondrial activity and frequency of oocytes with granulated mitochondria at GV and clustered mitochondria at MII than the SF subpopulation. With regard to atresia, mitochondrial activity decreased from healthy to mid-atretic oocytes in both MF and SF subpopulations at GV, but in the SF subpopulation at MII, the mitochondrial activity and frequency of oocytes with clustered mitochondria were significantly higher in light-atretic than in healthy oocytes. The light-atretic oocytes also produced more ATP than healthy ones in both SF and MF subpopulations. However, a significantly higher relative abundance of mRNA TFAM was found in SF than MF subpopulations at GV, and this difference remained in mid-atretic oocytes at MII. It can be concluded that meiotic competence and atresia level influence mitochondrial status of immature bovine oocytes. After maturation, healthy oocytes from medium follicles and light-atretic oocytes from small follicles were more developed in terms of mitochondrial status than the other oocytes.
Pracoviště	Ústav živočišné fyziologie a genetiky
Kontakt	Jana Zásmětová, knihovna@iapg.cas.cz, Tel.: 315 639 554
Rok sběru	2013

Počet záznamů: 1