Počet záznamů: 1
Delivery of Large Heterologous Polypeptides across the Cytoplasmic Membrane of Antigen-Presenting Cells by the Bordetella RTX Hemolysin Moiety Lacking the Adenylyl Cyclase Domain
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SYSNO ASEP 0378997 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Delivery of Large Heterologous Polypeptides across the Cytoplasmic Membrane of Antigen-Presenting Cells by the Bordetella RTX Hemolysin Moiety Lacking the Adenylyl Cyclase Domain Tvůrce(i) Holubová, Jana (MBU-M) RID, ORCID
Kamanová, Jana (MBU-M) ORCID, RID
Jelínek, J. (CZ)
Tomala, Jakub (MBU-M) RID, ORCID
Mašín, Jiří (MBU-M) RID, ORCID
Kosová, Martina (MBU-M)
Staněk, Ondřej (MBU-M) RID, ORCID
Bumba, Ladislav (MBU-M) RID, ORCID
Michálek, J. (CZ)
Kovář, Marek (MBU-M) RID, ORCID
Šebo, Peter (MBU-M) RID, ORCIDZdroj.dok. Infection and Immunity. - : American Society for Microbiology - ISSN 0019-9567
Roč. 80, č. 3 (2012), s. 1181-1192Poč.str. 12 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova MHC CLASS-I ; ESCHERICHIA-COLI ; PRESENTATION PATHWAY Vědní obor RIV EE - Mikrobiologie, virologie CEP IAA500200914 GA AV ČR - Akademie věd GAP207/11/0717 GA ČR - Grantová agentura ČR GAP301/11/0325 GA ČR - Grantová agentura ČR 1M0506 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy 2B06161 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy CEZ AV0Z50200510 - MBU-M (2005-2011) UT WOS 000300621600031 DOI 10.1128/IAI.05711-11 Anotace The Bordetella adenylate cyclase toxin-hemolysin (CyaA; also called ACT or AC-Hly) targets CD11b-expressing phagocytes and translocates into their cytosol an adenylyl cyclase (AC) that hijacks cellular signaling by conversion of ATP to cyclic AMP (cAMP). Intriguingly, insertion of large passenger peptides removes the enzymatic activity but not the cell-invasive capacity of the AC domain. This has repeatedly been exploited for delivery of heterologous antigens into the cytosolic pathway of CD11b-expressing dendritic cells by CyaA/AC(-) toxoids, thus enabling their processing and presentation on major histocompatibility complex (MHC) class I molecules to cytotoxic CD8(+) T lymphocytes (CTLs). We produced a set of toxoids with overlapping deletions within the first 371 residues of CyaA and showed that the structure of the AC enzyme does not contain any sequences indispensable for its translocation across target cell membrane Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2013
Počet záznamů: 1