Počet záznamů: 1
Survey of several methods deproteinizing human plasma before and within the chloroformate-mediated treatment of amino/carboxylic acids quantitated by gas chromatography
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SYSNO ASEP 0377579 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Survey of several methods deproteinizing human plasma before and within the chloroformate-mediated treatment of amino/carboxylic acids quantitated by gas chromatography Tvůrce(i) Hušek, Petr (BC-A)
Švagera, Z. (CZ)
Hanzlíková, D. (CZ)
Šimek, Petr (BC-A) RID, ORCIDZdroj.dok. Journal of Pharmaceutical and Biomedical Analysis. - : Elsevier - ISSN 0731-7085
67-68, AUG-SEP (2012), s. 159-162Poč.str. 4 s. Jazyk dok. eng - angličtina Země vyd. NL - Nizozemsko Klíč. slova plasma (lipo)protein precipitation ; chloroformate-treated supernatant ; S-amino acids Vědní obor RIV CB - Analytická chemie, separace CEP NS9755 GA MZd - Ministerstvo zdravotnictví GAP206/10/2401 GA ČR - Grantová agentura ČR CEZ AV0Z50070508 - ENTU-I, BC-A (2005-2011) UT WOS 000305591900023 DOI 10.1016/j.jpba.2012.04.027 Anotace Trichloroacetic acid, perchloric acid, phosphotungstic acid, acetonitrile, methanol and some other organic solvents were evaluated for their ability to provide protein and lipid-free plasma supernatants. The residual proteins, total cholesterol and triacylglycerols were assayed in the supernatant on a Beckman Analyzer. The free cholesterol and the neutral lipids were further analyzed by means of high-temperature GC analysis. The conditions for the deproteinizing step were optimized for minimal lipoprotein disruption. Three plasma sulfur and aromatic amino acids were used as model compounds to evaluate compatibility of the precipitation methods with a subsequent methyl chloroformate-mediated derivatization and GC–MS analysis. The results of the total homocysteine assay matched well with that obtained using a commercial immunoassay. Precipitation with trichloroacetic acid has proven to be a method of choice for the analysis of the acido-basic analytes by GC–MS via chloroformates. Pracoviště Biologické centrum (od r. 2006) Kontakt Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Rok sběru 2013 Elektronická adresa http://www.sciencedirect.com/science/article/pii/S0731708512002245
Počet záznamů: 1