Počet záznamů: 1

Epigenetic changes at Il12rb2 and Tbx21 in relation to plasticity behavior of Th17 cells

SYSNO ASEP	0375132
Druh ASEP	J - Článek v odborném periodiku
Zařazení RIV	J - Článek v odborném periodiku
Poddruh J	Článek ve WOS
Název	Epigenetic changes at Il12rb2 and Tbx21 in relation to plasticity behavior of Th17 cells
Tvůrce(i)	Bending, D. (GB) Newland, S. (GB) Krejčí, Alena (BC-A)_{RID, ORCID} Phillips, J. M. (GB) Bray, S. (GB) Cooke, A. (GB)
Celkový počet autorů	6
Zdroj.dok.	Journal of Immunology. - : American Association of Immunologists - ISSN 0022-1767 Roč. 186, č. 6 (2011), s. 3373-3382
Poč.str.	10 s.
Jazyk dok.	eng - angličtina
Země vyd.	US - Spojené státy americké
Klíč. slova	epigenetic change
Vědní obor RIV	EB - Genetika a molekulární biologie
CEZ	AV0Z50070508 - ENTU-I, BC-A (2005-2011)
UT WOS	000287923500013
DOI	10.4049/jimmunol.1003216
Anotace	Plasticity within Th cell populations may play a role in enabling site-specific immune responses to infections while limiting tissue destruction. Epigenetic processes are fundamental to such plasticity; however, to date, most investigations have focused on in vitrogenerated T cells. In this study, we have examined the molecular mechanisms underpinning murine Th17 plasticity in vivo by assessing H3K4 and H3K27 trimethylation marks at Tbx21, Rorc, Il17a, Ifng, and Il12rb2 loci in purified ex vivo-isolated and in vitro-generated Th17 cells. Although both populations had largely comparable epigenetic signatures, including bivalent marks at Tbx21, freshly isolated ex vivo Th17 cells displayed restricted expression from Il12rb2 due to the presence of repressive chromatin modifications. This receptor, however, could be upregulated on isolated ex vivo Th17 cells after in vitro activation or by in vivo immunization and was augmented by the presence of IFN-g. Such activated cells could then be deviated toward a Th1-like profile. We show that IL-12 stimulation removes H3K27 trimethylation modifications at Tbx21/T-bet leading to enhanced T-bet expression with in vitro Th17 cells. Our study reveals important potential phenotypic differences between ex vivo- and in vitro-generated Th17 cells and provides mechanistic insight into Th17 cell plasticity.
Pracoviště	Biologické centrum (od r. 2006)
Kontakt	Dana Hypšová, eje@eje.cz, Tel.: 387 775 214
Rok sběru	2012

Počet záznamů: 1