Počet záznamů: 1

Recognition of peptidoglycan and beta-lactam antibiotics by the extracellular domain of the Ser/Thr protein kinase StkP from Streptococcus pneumoniae

SYSNO ASEP	0370932
Druh ASEP	J - Článek v odborném periodiku
Zařazení RIV	J - Článek v odborném periodiku
Poddruh J	Článek ve WOS
Název	Recognition of peptidoglycan and beta-lactam antibiotics by the extracellular domain of the Ser/Thr protein kinase StkP from Streptococcus pneumoniae
Tvůrce(i)	Maestro, B. (ES) Nováková, Linda (MBU-M)_RID Hesek, D. (US) Lee, M. (US) Leyva, E. (ES) Mobashery, S. (US) Sanz, J.M. (ES) Branny, Pavel (MBU-M)_{RID, ORCID}
Zdroj.dok.	FEBS Letters. - : Wiley - ISSN 0014-5793 Roč. 585, č. 2 (2011), s. 357-363
Poč.str.	7 s.
Jazyk dok.	eng - angličtina
Země vyd.	GB - Velká Británie
Klíč. slova	Signal transduction ; Penicillin-binding protein and Ser/Thr protein kinase-associated domain ; Peptidoglycan
Vědní obor RIV	CE - Biochemie
CEP	GP204/07/P082 GA ČR - Grantová agentura ČR
	GA204/08/0783 GA ČR - Grantová agentura ČR
	IAA600200801 GA AV ČR - Akademie věd
CEZ	AV0Z50200510 - MBU-M (2005-2011)
UT WOS	000286180300016
DOI	10.1016/j.febslet.2010.12.016
Anotace	The eukaryotic-type serine/threonine kinase StkP from Streptococcus pneumoniae is an important signal-transduction element that regulates the expression of numerous pneumococcal genes. We have expressed the extracellular C-terminal domain of StkP kinase (C-StkP), elaborated a three-dimensional structural model and performed a spectroscopical characterization of its structure and stability. Biophysical experiments show that C-StkP binds to synthetic samples of the cell wall peptidoglycan (PGN) and to beta-lactam antibiotics, which mimic the terminal portions of the PGN stem peptide. This is the first experimental report on the recognition of a minimal PGN unit by a PASTA-containing kinase, suggesting that non-crosslinked PGN may act as a signal for StkP function and pointing to this protein as an interesting target for beta-lactam antibiotics.
Pracoviště	Mikrobiologický ústav
Kontakt	Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231
Rok sběru	2012

Počet záznamů: 1