Počet záznamů: 1
Preparation of Metallochelating Microbubbles and Study on Their Site-Specific Interaction with rGFP-HisTag as a Model Protein
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SYSNO ASEP 0370417 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Preparation of Metallochelating Microbubbles and Study on Their Site-Specific Interaction with rGFP-HisTag as a Model Protein Tvůrce(i) Lukáč, R. (CZ)
Kauerová, Z. (CZ)
Mašek, J. (CZ)
Bartheldyová, E. (CZ)
Kulich, P. (CZ)
Koudelka, Š. (CZ)
Korvasová, Z. (CZ)
Plocková, J. (CZ)
Papoušek, František (FGU-C)
Kolář, František (FGU-C) RID, ORCID, SAI
Schmidt, R. (DE)
Turánek, J. (CZ)Zdroj.dok. Langmuir. - : American Chemical Society - ISSN 0743-7463
Roč. 27, č. 8 (2011), s. 4829-4837Poč.str. 9 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova microbubble ; ultrasound imaging ; metallochelating bond ; rGFP ; liposome ; contrast echocardiography ; static light scattering ; flow ; cytometry ; confocal Vědní obor RIV FA - Kardiovaskulární nemoci vč. kardiochirurgie CEP KAN200520703 GA AV ČR - Akademie věd CEZ AV0Z50110509 - FGU-C (2005-2011) UT WOS 000289321000071 DOI 10.1021/la104677b Anotace Construction of surface-modified metallochelating microbubbles (MBs) was proved using recombinant green fluorescent protein (rGFP) with 6 HisTags. MBs composed of DPPC with various contents of metallochelating lipid DOGS-NTA-Ni were prepared by intensive shaking of liposome suspension under atmosphere of sulfur hexafluoride by 3M ESPE CapMix device. Static light scattering, flow cytometry and optical microscopy were used to determine mean size (2.1-2.8 μm) and concentration of MBs (109/mL). MBs were lyophilized in the presence of sucrose. These MBs were stable in vitro (half-live of MBs in bovine serum at 37 °C was 3-7 min) and in vivo (mouse model). DPPC-based metallochelating MBs provided clear and very contrast image of the ventricular cavity in mouse heart soon after injection as observed by ultrasonic system. Commercial MBs (Bracco) were used as standard. Site selective and stable binding of rGFP-HisTag onto the surface of metallochelating MBs was observed by confocal microscopy Pracoviště Fyziologický ústav Kontakt Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Rok sběru 2012
Počet záznamů: 1