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In vitro differentiation of mouse embryonic stem cells into neurons of the dorsal forebrain
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SYSNO ASEP 0370061 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název In vitro differentiation of mouse embryonic stem cells into neurons of the dorsal forebrain Tvůrce(i) Jing, Y. (NO)
Machoň, Ondřej (UMG-J) RID
Hampl, Aleš (UEM-P)
Dvořák, P. (CZ)
Xing, Y. (CN)
Krauss, S. (NO)Zdroj.dok. Cellular and Molecular Neurobiology. - : Springer - ISSN 0272-4340
Roč. 31, č. 5 (2011), s. 715-727Poč.str. 13 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova embryonic stem cells ; differentiation ; neocortex Vědní obor RIV EB - Genetika a molekulární biologie CEP GA204/08/1618 GA ČR - Grantová agentura ČR CEZ AV0Z50520514 - UMG-J (2005-2011) AV0Z50390512 - UEM-P (2005-2011) UT WOS 000290914300008 DOI 10.1007/s10571-011-9669-2 Anotace Pluripotent embryonic stem cells (ESCs) are able to differentiate into all cell types in the organism including neocortical neurons. To follow the dynamic generation of progenitors of the dorsal forebrain in vitro, we generated ESCs from D6-GFP mice in which GFP marks neocortical progenitors and neurons. Several differentiation protocols were used. GFP-positive neocorical cells were induced under differentiation conditions in quickly formed embryoid bodies (qEBs) after 12-day incubation. In contrast, protocols using normal embryoid bodies (nEBs) yielded only a few D6-GFP-positive cells. As shown by immunohistochemistry and qRT-PCR, neocortical cells generated from qEBs expressed characteristic genes such as Pax6, Dach1, Tbr1, Tbr2, or Sox5. Activation of Wnt signaling further stimulated generation of D6-GFP-positive cells. qEBs culture also allowed the formation of pseudo-polarized neuroepithelium with the apical N-cadherin and basal Pax6 that is typical of developing neocortex. Pracoviště Ústav molekulární genetiky Kontakt Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217 Rok sběru 2012
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