Počet záznamů: 1
Time-Dependent Oxidation during Nano-Assisted Laser Desorption Ionization Mass Spectrometry: A Useful Tool for Structure Determination or a Source of Possible Confusion?
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SYSNO ASEP 0369749 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Time-Dependent Oxidation during Nano-Assisted Laser Desorption Ionization Mass Spectrometry: A Useful Tool for Structure Determination or a Source of Possible Confusion? Tvůrce(i) Pavlásková, Kateřina (MBU-M)
Strnadová, Marcela (MBU-M)
Strohalm, Martin (MBU-M)
Havlíček, Vladimír (MBU-M) RID, ORCID
Šulc, Miroslav (MBU-M) RID, ORCID
Volný, Michael (MBU-M) ORCIDZdroj.dok. Analytical Chemistry. - : American Chemical Society - ISSN 0003-2700
Roč. 83, č. 14 (2011), s. 5661-5665Poč.str. 5 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova DOUBLE-BOND POSITION ; UNSATURATED FATTY-ACIDS ; ELECTROSPRAY-IONIZATION Vědní obor RIV CE - Biochemie CEP GPP206/10/P018 GA ČR - Grantová agentura ČR LC545 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy ME10013 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy CEZ AV0Z50200510 - MBU-M (2005-2011) UT WOS 000292892000026 DOI 10.1021/ac200801t Anotace This work reports on a new and extremely simple approach for determination of a double bond position by a laser desorption ionization mass spectrometry. It is solely based on the catalytic properties of nanostructured surfaces used in nanoassisted laser desorption ionization experiments. These surfaces can induce oxidation of analytes, which results in a mass shift that can be detected by mass spectrometry. If a site of unsaturation is oxidized and cleaved, the m/z difference is diagnostic of the position of a double bond. By demonstrating that the oxidation depends on the analyte surface dwell time, it was proven that it is caused by the surface activity and not by the laser desorption ionization process itself. Control matrix-assisted laser desorption/ionization (MALDI)experiment showed only a limited partial oxidation and no time dependency of the process Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2012
Počet záznamů: 1