Počet záznamů: 1
Ligand-binding properties of a juvenile hormone receptor, Methoprene-tolerant
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SYSNO ASEP 0369355 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Ligand-binding properties of a juvenile hormone receptor, Methoprene-tolerant Tvůrce(i) Charles, J.-P. (FR)
Iwema, T. (FR)
Epa, V. C. (AU)
Takaki, Keiko (BC-A)
Ryneš, J. (CZ)
Jindra, Marek (BC-A) RID, ORCIDCelkový počet autorů 6 Zdroj.dok. Proceedings of the National Academy of Sciences of the United States of America. - : National Academy of Sciences - ISSN 0027-8424
Roč. 108, č. 52 (2011), s. 21128-21133Poč.str. 6 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova structure modeling ; insecticide action ; metamorphosis Vědní obor RIV EB - Genetika a molekulární biologie CEP GD204/09/H058 GA ČR - Grantová agentura ČR GA204/07/1032 GA ČR - Grantová agentura ČR IAA500960906 GA AV ČR - Akademie věd CEZ AV0Z50070508 - ENTU-I, BC-A (2005-2011) UT WOS 000298479900054 DOI 10.1073/pnas.1116123109 Anotace Juvenile hormone (JH) is a terpenoid, a small lipophilic molecule of vital importance to insects. The molecular action of JH has for long decades remained an enigma, because a JH receptor could not be identified. In this study we provide conclusive evidence for the JH receptor role of the bHLH-PAS domain transcription factor Methoprene-tolerant (Met). By testing Met point mutant proteins in vitro, we determined specific amino acids contributing to the high-affinity JH binding. Using mutants unable to bind JH, we demonstrated that interactions of Met with partner proteins require the ligand-binding capacity of Met. Therefore, Met resembles certain nuclear receptors, e. g., of retinoic acid or thyroid hormone, but since Met belongs to a different protein family, it establishes a prototype of a hormonal receptor of a novel class. Our results address a long-standing question of the mechanism of action of juvenile hormone and its insecticidal analogs. Pracoviště Biologické centrum (od r. 2006) Kontakt Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Rok sběru 2012
Počet záznamů: 1