Počet záznamů: 1

Limitations of Electronic Energy Transfer in the Determination of Lipid Nanodomain Sizes

SYSNO ASEP	0369083
Druh ASEP	J - Článek v odborném periodiku
Zařazení RIV	J - Článek v odborném periodiku
Poddruh J	Článek ve WOS
Název	Limitations of Electronic Energy Transfer in the Determination of Lipid Nanodomain Sizes
Tvůrce(i)	Šachl, Radek (UFCH-W)_{RID, ORCID} Humpolíčková, Jana (UFCH-W)_RID Štefl, Martin (UFCH-W) Johansson, L. B. A. (SE) Hof, Martin (UFCH-W)_{RID, ORCID}
Zdroj.dok.	Biophysical Journal. - : Cell Press - ISSN 0006-3495 Roč. 101, č. 11 (2011), L60-L62
Poč.str.	3 s.
Jazyk dok.	eng - angličtina
Země vyd.	US - Spojené státy americké
Klíč. slova	Foerster resonance energy transfer ; nanodomains ; Monte Carlo Setup
Vědní obor RIV	CF - Fyzikální chemie a teoretická chemie
CEP	GAP208/10/1090 GA ČR - Grantová agentura ČR
	GAP208/10/0376 GA ČR - Grantová agentura ČR
CEZ	AV0Z40400503 - UFCH-W (2005-2011)
UT WOS	000297897300002
DOI	10.1016/j.bpj.2011.11.001
Anotace	Even though superresolution microscopy indicates that size of plasma membrane rafts is <20 nm, those structures have never been observed. Förster resonance energy transfer (FRET) is therefore still the most powerful optical method for characterization of such domains. In this letter we investigate relation between nanodomain affinity of a donor-acceptor (D/A) pair and the detectable nanodomain size/area. We show that probes with high affinity to the liquid-ordered (Lo) phase are required for detecting domain sizes of a few nanometers, and/or domains that occupy a few percent of the bilayer area. A combination of donors and acceptors that prefer different phases is the more favorable approach. For instance, a D/A pair with the distribution constant of donors KD = 5 and acceptors KA = 0.01 can resolve a broad spectrum of nanodomain sizes. On the other hand, currently available donors and acceptors that prefer the same phase, either the liquid-disordered (Ld) or Lo phase, are not so convenient for determining domain sizes <20 nm. Here the detection limits of FRET experiments employing several commonly used D/A pairs have been investigated.
Pracoviště	Ústav fyzikální chemie J.Heyrovského
Kontakt	Michaela Knapová, michaela.knapova@jh-inst.cas.cz, Tel.: 266 053 196
Rok sběru	2012

Počet záznamů: 1