Počet záznamů: 1
Analysis of blastomogenic activity of mammal carcinogens in Drosophila using the wts (P4) allele and RNA interference-induced P53 silencing
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SYSNO ASEP 0368651 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Analysis of blastomogenic activity of mammal carcinogens in Drosophila using the wts (P4) allele and RNA interference-induced P53 silencing Tvůrce(i) Kirsanov, K. I. (RU)
Lesovaya, E. A. (RU)
Sidorov, Roman (BC-A) RID
Belitsky, G. A. (RU)
Yakubovskaya, M. G. (RU)Celkový počet autorů 5 Zdroj.dok. Russian Journal of Genetics - ISSN 1022-7954
Roč. 47, č. 4 (2011), s. 409-416Poč.str. 8 s. Jazyk dok. eng - angličtina Země vyd. RU - Rusko Klíč. slova Drosophila Vědní obor RIV EB - Genetika a molekulární biologie CEP IAA500070601 GA AV ČR - Akademie věd CEZ AV0Z50070508 - ENTU-I, BC-A (2005-2011) UT WOS 000289574400004 DOI 10.1134/S1022795411040065 Anotace The test for somatic mutagenesis and recombination in Drosophila is one of the widely used approaches for determination of possible carcinogenic effects of chemical compounds. The use of heterozygotes for mutant tumor suppressor gene wts enables more direct evaluation of the blastomogenic effects of chemical compounds, by tumor formation in the adult flies. This study presents evaluation of the SMART effectiveness upon the use of Drosophila heterozygotes for the wts (P4) gene, first included into the test system. The increase of the test resolution capacity compared to the literature data for the wts (P2) allele was observed. Using wts (P4) heterozygotes, a total of 20 carcinogenic compounds, and their slightly carcinogenic and non-carcinogenic analogs were tested. Specificity of the method was about 100%, and sensitivity depended on the type of the agent tested. The latter was absolute for the direct action carcinogens, with respect to carcinogens, requiring the metabolic activation. Pracoviště Biologické centrum (od r. 2006) Kontakt Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Rok sběru 2012
Počet záznamů: 1