Počet záznamů: 1

Chromosome Isolation by Flow Sorting in Aegilops umbellulata and Ae. comosa and Their Allotetraploid Hybrids Ae. biuncialis and Ae. geniculata

SYSNO ASEP	0368586
Druh ASEP	J - Článek v odborném periodiku
Zařazení RIV	J - Článek v odborném periodiku
Poddruh J	Článek ve WOS
Název	Chromosome Isolation by Flow Sorting in Aegilops umbellulata and Ae. comosa and Their Allotetraploid Hybrids Ae. biuncialis and Ae. geniculata
Tvůrce(i)	Molnár, I. (HU) Kubaláková, Marie (UEB-Q)_RID Šimková, Hana (UEB-Q)_{RID, ORCID} Cseh, A. (HU) Molnár-Láng, M. (HU) Doležel, Jaroslav (UEB-Q)_{RID, ORCID}
Zdroj.dok.	PLoS ONE. - : Public Library of Science - ISSN 1932-6203 Roč. 66, č. 11 (2011), s. 1-11
Poč.str.	11 s.
Jazyk dok.	eng - angličtina
Země vyd.	US - Spojené státy americké
Klíč. slova	wheat ; chromosome isolation ; Aegilops umbellulata
Vědní obor RIV	EB - Genetika a molekulární biologie
CEP	LC06004 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy
	OC08025 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy
CEZ	AV0Z50380511 - UEB-Q (2005-2011)
UT WOS	000298162000035
DOI	10.1371/journal.pone.0027708
Anotace	This study evaluates the potential of flow cytometry for chromosome sorting in two wild diploid wheats Aegilops umbellulata and Ae. comosa and their natural allotetraploid hybrids Ae. biuncialis and Ae. geniculata. Flow karyotypes obtained after the analysis of DAPI-stained chromosomes were characterized and content of chromosome peaks was determined. Peaks of chromosome 1U could be discriminated in flow karyotypes of Ae. umbellulata and Ae. biuncialis and the chromosome could be sorted with purities exceeding 95%. The remaining chromosomes formed composite peaks and could be sorted in groups of two to four. Twenty four wheat SSR markers were tested for their position on chromosomes of Ae. umbellulata and Ae. comosa using PCR on DNA amplified from flow-sorted chromosomes and genomic DNA of wheat-Ae. geniculata addition lines, respectively. Six SSR markers were located on particular Aegilops chromosomes using sorted chromosomes, thus confirming the usefulness of this approach for physical mapping.
Pracoviště	Ústav experimentální botaniky
Kontakt	David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469
Rok sběru	2012

Počet záznamů: 1