Počet záznamů: 1
Changes in cytokinin levels and metabolism in tobacco (Nicotiana tabacum L.) explants during in vitro shoot organogenesis induced by trans-zeatin and dihydrozeatin
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SYSNO ASEP 0368520 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Changes in cytokinin levels and metabolism in tobacco (Nicotiana tabacum L.) explants during in vitro shoot organogenesis induced by trans-zeatin and dihydrozeatin Tvůrce(i) Klemš, M. (CZ)
Slámová, Z. (CZ)
Motyka, Václav (UEB-Q) RID, ORCID
Malbeck, Jiří (UEB-Q) RID, ORCID
Trávníčková, Alena (UEB-Q) ORCID
Macháčková, Ivana (UEB-Q)
Holík, Josef (UEB-Q) RID, ORCID
Procházka, S. (CZ)Zdroj.dok. Plant Growth Regulation. - : Springer - ISSN 0167-6903
Roč. 65, č. 3 (2011), s. 427-437Poč.str. 11 s. Jazyk dok. eng - angličtina Země vyd. NL - Nizozemsko Klíč. slova Beta-glucosidase ; Cytokinin oxidase/ dehydrogenase ; Dihydrozeatin Vědní obor RIV EB - Genetika a molekulární biologie CEP 1M06030 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy GAP506/11/0774 GA ČR - Grantová agentura ČR IAA600380701 GA AV ČR - Akademie věd CEZ AV0Z50380511 - UEB-Q (2005-2011) UT WOS 000297621200001 DOI 10.1007/s10725-011-9612-z Anotace The uptake and metabolism of trans-zeatin (Z) and/or dihydrozeatin (DHZ), in correlation with cytokinin oxidase/dehydrogenase (CKX) and glucosidase activity, were studied in leaf segments derived from wild-type (WT) and transgenic (T) tobacco (Nicotiana tabacum L. cv. Petit Havana SR1) during in vitro induction of shoot organogenesis. T explants harbored the maize gene Zm-p60.1-glucosidase. Higher levels of shoot regeneration were observed on T explants in the early stages of cultivation. In WT explants, the content of cytokinin (CK)-O- and N-glucosides increased. In T explants, a higher content of Z-9-riboside and its monophosphate and higher CKX activity during the early stage of cultures were found. A positive correlation was obtained for bioactive CK content and the organogenic response in T explants. Our results indicate a connection between the organogenic capacity of tobacco explants, metabolism of endogenous CKs and uptake of exogenous CKs from the cultivation medium. Pracoviště Ústav experimentální botaniky Kontakt David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Rok sběru 2012
Počet záznamů: 1