Počet záznamů: 1
Activation of Cumulus Cell SMAD2/3 and Epidermal Growth Factor Receptor Pathways Are Involved in Porcine Oocyte-Cumulus Cell Expansion and Steroidogenesis
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SYSNO ASEP 0368348 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Activation of Cumulus Cell SMAD2/3 and Epidermal Growth Factor Receptor Pathways Are Involved in Porcine Oocyte-Cumulus Cell Expansion and Steroidogenesis Tvůrce(i) Nagyová, Eva (UZFG-Y) RID, ORCID
Camaioni, A. (IT)
Scsuková, S. (SK)
Mlynarčíková, A. (SK)
Procházka, Radek (UZFG-Y) RID, ORCID
Němcová, Lucie (UZFG-Y) RID
Salustri, A. (IT)Celkový počet autorů 7 Zdroj.dok. Molecular Reproduction and Development - ISSN 1040-452X
Roč. 78, č. 6 (2011), s. 391-402Poč.str. 12 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova MURAL GRANULOSA-CELLS ; IN-VITRO MATURATION ; PREOVULATORY OVARIAN-FOLLICLES Vědní obor RIV EB - Genetika a molekulární biologie CEP GA523/08/0111 GA ČR - Grantová agentura ČR CEZ AV0Z50450515 - UZFG-Y (2005-2011) UT WOS 000292500700003 DOI 10.1002/mrd.21312 Anotace Several lines of evidence suggest that in mice the activation of SMAD2/3 signaling by oocyte secreted factors, together with epidermal growth factor receptor (EGFR) activation, is essential to induce cumulus expansion. Here we show that inhibition of EGFR kinase in follicle stimulating hormone (FSH)-stimulated porcine oocyte-cumulus cell complex (OCCs) strongly decreases hyaluronan (HA) synthesis and its retention in the matrix, as well as progesterone synthesis. Although porcine cumulus cells undergo expansion independently of oocytes, we use biochemical and gene expression analyses to show that they do require activation of SMAD2/3 for optimal stimulation of HA synthesis and proteins involved in the organization of this polymer in the expanded matrix. Furthermore, FSH-induced progesterone synthesis by porcine cumulus cells was increased by blocking SMAD2/3 activation. Pracoviště Ústav živočišné fyziologie a genetiky Kontakt Jana Zásmětová, knihovna@iapg.cas.cz, Tel.: 315 639 554 Rok sběru 2012
Počet záznamů: 1