Počet záznamů: 1

Raster image correlation spectroscopy as a novel tool to study interactions of macromolecules with nanofiber scaffolds

SYSNO ASEP	0368065
Druh ASEP	J - Článek v odborném periodiku
Zařazení RIV	J - Článek v odborném periodiku
Poddruh J	Článek ve WOS
Název	Raster image correlation spectroscopy as a novel tool to study interactions of macromolecules with nanofiber scaffolds
Tvůrce(i)	Norris, S. C. P. (US) Humpolíčková, Jana (UFCH-W)_RID Amler, Evžen (UEM-P)_RID Huranová, Martina (UMG-J)_ORCID Buzgo, Matej (UEM-P) Macháň, Radek (UFCH-W) Lukáš, D. (CZ) Hof, Martin (UFCH-W)_{RID, ORCID}
Zdroj.dok.	Acta Biomaterialia. - : Elsevier - ISSN 1742-7061 Roč. 7, č. 12 (2011), s. 4195-4203
Poč.str.	9 s.
Jazyk dok.	eng - angličtina
Země vyd.	GB - Velká Británie
Klíč. slova	fluorecence dynamics ; transient binding ; diffusion
Vědní obor RIV	CF - Fyzikální chemie a teoretická chemie
CEP	LC06063 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy
	IAA500390702 GA AV ČR - Akademie věd
	GAP304/10/1307 GA ČR - Grantová agentura ČR
CEZ	AV0Z40400503 - UFCH-W (2005-2011)
	AV0Z50390512 - UEM-P (2005-2011)
	AV0Z50390703 - UEM-P (2007-2013)
	AV0Z50520514 - UMG-J (2005-2011)
UT WOS	000297436500012
DOI	10.1016/j.actbio.2011.07.012
Anotace	Dynamic processes such as diffusion and binding/unbinding of macromolecules (e.g. growth factors or nutrients) are crucial parameters for the design and application of effective artificial tissue materials. Here, dynamics of selected macromolecules were studied in two different composite tissue engineering scaffolds containing an electrospun nanofiber mesh (polycaprolactone or hydrophobically plasma modified polyvinylalcohol–chitosan) encapsulated in agarose hydrogels by a conventional approach fluorescence recovery after photobleaching (FRAP) and a novel technique, raster image correlation spectroscopy (RICS). The two approaches are compared, and it is shown that FRAP is unable to determine processes occurring at low molecular concentrations, especially accurately separating binding/unbinding from diffusion, and its results depend on the concentration of the studied molecules.
Pracoviště	Ústav fyzikální chemie J.Heyrovského
Kontakt	Michaela Knapová, michaela.knapova@jh-inst.cas.cz, Tel.: 266 053 196
Rok sběru	2012

Počet záznamů: 1