Počet záznamů: 1
Raster image correlation spectroscopy as a novel tool to study interactions of macromolecules with nanofiber scaffolds
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SYSNO ASEP 0368065 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Raster image correlation spectroscopy as a novel tool to study interactions of macromolecules with nanofiber scaffolds Tvůrce(i) Norris, S. C. P. (US)
Humpolíčková, Jana (UFCH-W) RID
Amler, Evžen (UEM-P) RID
Huranová, Martina (UMG-J) ORCID
Buzgo, Matej (UEM-P)
Macháň, Radek (UFCH-W)
Lukáš, D. (CZ)
Hof, Martin (UFCH-W) RID, ORCIDZdroj.dok. Acta Biomaterialia. - : Elsevier - ISSN 1742-7061
Roč. 7, č. 12 (2011), s. 4195-4203Poč.str. 9 s. Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova fluorecence dynamics ; transient binding ; diffusion Vědní obor RIV CF - Fyzikální chemie a teoretická chemie CEP LC06063 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy IAA500390702 GA AV ČR - Akademie věd GAP304/10/1307 GA ČR - Grantová agentura ČR CEZ AV0Z40400503 - UFCH-W (2005-2011) AV0Z50390512 - UEM-P (2005-2011) AV0Z50390703 - UEM-P (2007-2013) AV0Z50520514 - UMG-J (2005-2011) UT WOS 000297436500012 DOI https://doi.org/10.1016/j.actbio.2011.07.012 Anotace Dynamic processes such as diffusion and binding/unbinding of macromolecules (e.g. growth factors or nutrients) are crucial parameters for the design and application of effective artificial tissue materials. Here, dynamics of selected macromolecules were studied in two different composite tissue engineering scaffolds containing an electrospun nanofiber mesh (polycaprolactone or hydrophobically plasma modified polyvinylalcohol–chitosan) encapsulated in agarose hydrogels by a conventional approach fluorescence recovery after photobleaching (FRAP) and a novel technique, raster image correlation spectroscopy (RICS). The two approaches are compared, and it is shown that FRAP is unable to determine processes occurring at low molecular concentrations, especially accurately separating binding/unbinding from diffusion, and its results depend on the concentration of the studied molecules. Pracoviště Ústav fyzikální chemie J.Heyrovského Kontakt Michaela Knapová, michaela.knapova@jh-inst.cas.cz, Tel.: 266 053 196 Rok sběru 2012
Počet záznamů: 1