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Trypanosoma brucei Mitochondrial Respiratome: Composition and Organization in Procyclic Form
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SYSNO ASEP 0367704 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Trypanosoma brucei Mitochondrial Respiratome: Composition and Organization in Procyclic Form Tvůrce(i) Acestor, N. (US)
Zíková, Alena (BC-A) RID, ORCID
Dalley, R. A. (US)
Anupama, A. (US)
Panigrahi, A. K. (US)
Stuart, K. D. (US)Zdroj.dok. Molecular & Cellular Proteomics. - : Elsevier - ISSN 1535-9476
Roč. 10, č. 9 (2011), s. 1-14Poč.str. 14 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova SUCCINATE DEHYDROGENASE ; EDITED MESSENGER-RNA ; COMPLEX-I ; TRYPANOSOMA-BRUCEI ; UBIQUINONE OXIDOREDUCTASE ; TAP-TAG ; PROTEIN INTERACTION ; ALTERNATIVE OXIDASE ; STATISTICAL-MODEL ; MASS-SPECTROMETRY Vědní obor RIV EB - Genetika a molekulární biologie CEP GP204/09/P563 GA ČR - Grantová agentura ČR CEZ AV0Z60220518 - PAU-O, BC-A (2005-2011) UT WOS 000294729200007 DOI 10.1074/mcp.M110.006908 Anotace The mitochondrial respiratory chain is comprised of four different protein complexes (I-IV), which are responsible for electron transport and generation of proton gradient in the mitochondrial intermembrane space. In this study, the respiratory complexes I, II, and III were affinity purified from Trypanosoma brucei procyclic form cells and their composition was determined by mass spectrometry. The results showed that the respiratome of Trypanosoma is divergent because many of its proteins are unique to this group of organisms. Proteomics data from analyses of complexes purified using numerous tagged component proteins in each of the five complexes were used to generate the first predicted protein-protein interaction network of the Trypanosoma brucei respiratory chain. These results provide the first comprehensive insight into the unique composition of the respiratory complexes in Trypanosoma brucei, an early diverged eukaryotic pathogen. Pracoviště Biologické centrum (od r. 2006) Kontakt Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Rok sběru 2012
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