Počet záznamů: 1
Ubiquitin-activating enzyme (UBA1) is required for sperm capacitation, acrosomal exocytosis and sperm-egg coat penetration during porcine fertilization
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SYSNO ASEP 0367613 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Ubiquitin-activating enzyme (UBA1) is required for sperm capacitation, acrosomal exocytosis and sperm-egg coat penetration during porcine fertilization Tvůrce(i) Yi, Y.-J. (KR)
Zimmermann, S.W. (US)
Manandhar, G. (US)
Odhiambo, J.F. (US)
Kennedy, C. (US)
Jonáková, Věra (BTO-N) RID
Maňásková-Postlerová, Pavla (BTO-N) ORCID
Sutovsky, M. (US)
Park, C.-S. (US)
Sutovsky, P. (US)Celkový počet autorů 10 Zdroj.dok. International Journal of Andrology. - : WILEY-BLACKWELL - ISSN 0105-6263
Roč. 35, č. 2 (2012), s. 196-210Poč.str. 15 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova acrosome ; capacitation ; fertilization ; ubiqutin Vědní obor RIV CE - Biochemie CEP GA303/09/1285 GA ČR - Grantová agentura ČR 1M06011 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy NS10009 GA MZd - Ministerstvo zdravotnictví CEZ AV0Z50520701 - BTO-N (2007-2013) UT WOS 000301442200012 DOI 10.1111/j.1365-2605.2011.01217.x. Anotace Protein ubiquitination is covalent post-translational modification that targets proteins for proteolysis by the 26S proteasome. The E1-type ubiquitin-activating enzyme (UBA1) is responsible for the initial step of ubiquitin–protein ligation. Proteasomal proteolysis of ubiquitinated spermatozoa and oocyte proteins occurs during mammalian fertilization. UBA1 was detected in boar sperm-acrosomal extracts by Western blotting (WB) and by IMF in the acrosomal caps. A specific UBA1 inhibitor, PYR-41, altered the outer acrosomal membrane during sperm capacitation and reduced fertilization rates during in vitro fertilization. WB with antiphosphotyrosine antibodies and antibodies against acrosomal proteins SPINK2 (acrosin inhibitor) and AQN1 (spermadhesin) revealed that the capacitationinduced modification of those proteins was altered by PYR-41. It appears that de novo protein ubiquitination involving UBA1 contributes to sperm capacitation and acrosomal function during fertilization. Pracoviště Biotechnologický ústav Kontakt Monika Kopřivová, Monika.Koprivova@ibt.cas.cz, Tel.: 325 873 700 Rok sběru 2013
Počet záznamů: 1