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The phosphomimetic mutation of an evolutionarily conserved serine residue affects the signaling properties of Rho of plants (ROPs)
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SYSNO ASEP 0367374 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název The phosphomimetic mutation of an evolutionarily conserved serine residue affects the signaling properties of Rho of plants (ROPs) Tvůrce(i) Fodor-Dunai, C. (HU)
Fricke, I. (DE)
Potocký, Martin (UEB-Q) RID, ORCID
Dorjgotov, D. (HU)
Domoki, M. (DE)
Jurca, M. E. (DE)
Oetvoes, K. (HU)
Žárský, Viktor (UEB-Q) RID, ORCID
Berken, A. (DE)
Feher, A. (HU)Zdroj.dok. Plant Journal. - : Wiley - ISSN 0960-7412
Roč. 66, č. 4 (2011), s. 669-679Poč.str. 11 s. Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova Rho GTPase ; plant-specific ROP nucleotide exchanger ; ROP guanine nucleotide exchange factor Vědní obor RIV EB - Genetika a molekulární biologie CEP GP522/09/P299 GA ČR - Grantová agentura ČR CEZ AV0Z50380511 - UEB-Q (2005-2011) UT WOS 000290456400009 DOI 10.1111/j.1365-313X.2011.04528.x Anotace Plant ROP proteins form a unique subgroup of Rho-type small G-proteins. Here we demonstrate that the phosphomimetic mutation of a serine conserved in all Rho proteins affects ROP signaling. We found that the S74E mutation prevented the binding of Medicago ROP6 to its upstream activator, PRONE-domain-containing RopGEF protein and abolished the PRONE-mediated nucleotide exchange in vitro. Structural modeling supported the hypothesis that phosphorylation of the S74 residue interferes with the binding of the PRONE-domain to the adjacent R76 residue which is critical in ROP-PRONE interaction. Moreover, we show that the capability of S74E mutant to activate the RRK1 kinase was reduced. This correlates with the morphology of tobacco pollen tubes expressing mutant forms of YFP:ROP6. The S74E mutation had no influence on pollen tube morphology. The presented data suggest that the phosphorylation of the serine corresponding to S74 in ROP6 is a general principle for regulating ROP signaling. Pracoviště Ústav experimentální botaniky Kontakt David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Rok sběru 2012
Počet záznamů: 1