Počet záznamů: 1

Antiviral activity of tenofovir against Cauliflower mosaic virus and its metabolism in Brassica pekinensis plants

SYSNO ASEP	0367346
Druh ASEP	J - Článek v odborném periodiku
Zařazení RIV	J - Článek v odborném periodiku
Poddruh J	Článek ve WOS
Název	Antiviral activity of tenofovir against Cauliflower mosaic virus and its metabolism in Brassica pekinensis plants
Tvůrce(i)	Špak, Josef (BC-A)_{RID, ORCID} Votruba, Ivan (UOCHB-X)_RID Pavingerová, Daniela (BC-A) Holý, Antonín (UOCHB-X) Špaková, Vlastimila (BC-A) Petrzik, Karel (BC-A)_{RID, ORCID}
Celkový počet autorů	6
Zdroj.dok.	Antiviral Research. - : Elsevier - ISSN 0166-3542 Roč. 92, č. 2 (2011), s. 378-381
Poč.str.	4 s.
Jazyk dok.	eng - angličtina
Země vyd.	NL - Nizozemsko
Klíč. slova	Caulimovirus ; dsDNA ; Brassica
Vědní obor RIV	CE - Biochemie
CEP	GA522/09/0707 GA ČR - Grantová agentura ČR
CEZ	AV0Z50510513 - UMBR-M, BC-A (2005-2011)
	AV0Z40550506 - UOCHB-X (2005-2011)
UT WOS	000297427200030
DOI	10.1016/j.antiviral.2011.08.014
Anotace	The antiviral effect of the acyclic nucleoside phosphonate tenofovir (R)-PMPA on double-stranded DNA Cauliflower mosaic virus (CaMV) in Brassica pekinensis plants grown in vitro on liquid medium was evaluated. Double antibody sandwich ELISA and PCR were used for relative quantification of viral protein and detecting nucleic acid in plants. (R)-PMPA at concentrations of 25 and 50 mg/l significantly reduced CaMV titers in plants within 6–9 weeks to levels detectable neither by ELISA nor by PCR. Virus-free plants were obtained after 3-month cultivation of meristem tips on semisolid medium containing 50 mg/l (R)-PMPA and their regeneration to whole plants in the greenhouse. Studying the metabolism of (R)-PMPA in B. pekinensis revealed that mono- and diphosphate, structural analogs of NDP and/or NTP, are the only metabolites formed. The data indicate very low substrate activity of the enzymes toward (R)-PMPA as substrate.
Pracoviště	Biologické centrum (od r. 2006)
Kontakt	Dana Hypšová, eje@eje.cz, Tel.: 387 775 214
Rok sběru	2012

Počet záznamů: 1