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Characterization of a new 4BS.7HL wheat–barley translocation line using GISH, FISH, and SSR markers and its effect on the β-glucan content of wheat
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SYSNO ASEP 0366032 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Characterization of a new 4BS.7HL wheat–barley translocation line using GISH, FISH, and SSR markers and its effect on the β-glucan content of wheat Tvůrce(i) Cseh, A. (HU)
Kruppa, K. (HU)
Molnár, I. (HU)
Rakszegi, M. (HU)
Doležel, Jaroslav (UEB-Q) RID, ORCID
Molnár-Láng, M. (HU)Zdroj.dok. Genome. - : Canadian Science Publishing - ISSN 0831-2796
Roč. 54, č. 10 (2011), s. 795-804Poč.str. 10 s. Jazyk dok. eng - angličtina Země vyd. CA - Kanada Klíč. slova wheat–barley chromosome translocation ; β-d-glucan ; centromere Vědní obor RIV EB - Genetika a molekulární biologie CEZ AV0Z50380511 - UEB-Q (2005-2011) UT WOS 000296987100001 DOI 10.1139/g11-044 Anotace A spontaneous interspecific Robertsonian translocation was revealed by genomic in situ hybridization (GISH) in the progenies of a monosomic 7H addition line originating from a new wheat ‘Asakaze komugi’ × barley ‘Manas’ hybrid. Fluorescence in situ hybridization (FISH) with repetitive DNA sequences (Afa family, pSc119.2, and pTa71) allowed identification of all wheat chromosomes, including wheat chromosome arm 4BS involved in the translocation. FISH using barley telomere- and centromere-specific repetitive DNA probes (HvT01 and (AGGGAG)n) confirmed that one of the arms of barley chromosome 7H was involved in the translocation. Simple sequence repeat (SSR) markers specific to the long (L) and short (S) arms of barley chromosome 7H identified the translocated chromosome segment as 7HL. The presence of the HvCslF6 gene, responsible for (1,3;1,4)-b-D-glucan production, was revealed in the centromeric region of 7HL. Pracoviště Ústav experimentální botaniky Kontakt David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Rok sběru 2012
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