Počet záznamů: 1
Stage-specific requirement for Isa1 and Isa2 proteins in the mitochondrion of Trypanosoma brucei and heterologous rescue by human and Blastocystis orthologues
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SYSNO ASEP 0365995 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Stage-specific requirement for Isa1 and Isa2 proteins in the mitochondrion of Trypanosoma brucei and heterologous rescue by human and Blastocystis orthologues Tvůrce(i) Long, Shaojun (BC-A)
Changmai, Piya (BC-A)
Tsaousis, A.D. (CA)
Skalický, Tomáš (BC-A)
Verner, Zdeněk (BC-A)
Wen, Yan-Zi (BC-A)
Roger, A. J. (CA)
Lukeš, Julius (BC-A) RID, ORCIDZdroj.dok. Molecular Microbiology - ISSN 0950-382X
Roč. 81, č. 6 (2011), 1403-1418Poč.str. 16 s. Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova IRON-SULFUR CLUSTER ; ESCHERICHIA-COLI ; ASSEMBLY PROTEIN ; SACCHAROMYCES-CEREVISIAE ; AZOTOBACTER-VINELANDII ; CYSTEINE DESULFURASE ; CRYSTAL-STRUCTURE ; BINDING ACTIVITY ; GENE-CLUSTER Vědní obor RIV EB - Genetika a molekulární biologie CEP GA204/09/1667 GA ČR - Grantová agentura ČR LC07032 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy 2B06129 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy CEZ AV0Z60220518 - PAU-O, BC-A (2005-2011) UT WOS 000295087000001 DOI 10.1111/j.1365-2958.2011.07769.x Anotace IscA/Isa proteins function as alternative scaffolds for the assembly of Fe-S clusters and/or provide iron for their assembly in prokaryotes and eukaryotes. We have studied their function in Trypanosoma brucei, where the requirement for both of them to sustain cell growth depends on the life cycle stage. Reactive oxygen species but not iron accumulate in the procyclic mitochondrion upon ablation of the TbIsa proteins, but their depletion does not influence the assembly of Fe-S clusters in cytosolic proteins. In the bloodstream form, which has a downregulated mitochondrion, the TbIsa proteins are non-essential. The Isa2 orthologue of the anaerobic protist Blastocystis partially rescued the growth and enzymatic activities of TbIsa1/2 knock-down. Rescues with human Isa orthologues partially recovered the activities of aconitase and fumarase. These results show that the Isa1 and Isa2 proteins of diverse eukaryotes have overlapping functions. Pracoviště Biologické centrum (od r. 2006) Kontakt Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Rok sběru 2012
Počet záznamů: 1