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A platform for efficient genotyping in Musa using microsatellite markers

SYSNO ASEP	0365578
Druh ASEP	J - Článek v odborném periodiku
Zařazení RIV	J - Článek v odborném periodiku
Poddruh J	Ostatní články
Název	A platform for efficient genotyping in Musa using microsatellite markers
Tvůrce(i)	Christelová, Pavla (UEB-Q)_{RID, ORCID} Valárik, Miroslav (UEB-Q)_{RID, ORCID} Hřibová, Eva (UEB-Q)_{RID, ORCID} Van den Houwe, I. (BE) Channeliere, S. (FR) Roux, N. (FR) Doležel, Jaroslav (UEB-Q)_{RID, ORCID}
Zdroj.dok.	AoB PLANTS. - : Oxford University Press - ISSN 2041-2851 Roč. 2011, - (2011), s. 1-14
Poč.str.	14 s.
Jazyk dok.	eng - angličtina
Země vyd.	GB - Velká Británie
Klíč. slova	Musa ; microsatellite markers ; Cassava
Vědní obor RIV	EB - Genetika a molekulární biologie
CEZ	AV0Z50380511 - UEB-Q (2005-2011)
DOI	10.1093/aobpla/plr024
Anotace	With the aim of providing a simple and robust approach for molecular characterization of Musa species, we developed an optimized genotyping platform using 19 published simple sequence repeat markers. The genotyping system is based on 19 microsatellite loci, which are scored using fluorescently labelled primers and high-throughput capillary electrophoresis separation with high resolution. This genotyping platform was tested and optimized on a set of 70 diploid and 38 triploid banana accessions. The marker set used in this study provided enough polymorphism to discriminate between individual species, subspecies and subgroups of all accessions of Musa. Likewise, the capability of identifying duplicate samples was confirmed. Based on the results of a blind test, the genotyping system was confirmed to be suitable for characterization of unknown accessions. Here we report on the first complex and standardized platform for molecular characterization of Musa germplasm.
Pracoviště	Ústav experimentální botaniky
Kontakt	David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469
Rok sběru	2012

Počet záznamů: 1