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A NOVEL APPROACH FOR THE DETERMINATION OF MEMBRANE PROTEIN EXPRESSION LEVELS BASED ON LC-MS/MS

SYSNO ASEP	0365269
Druh ASEP	J - Článek v odborném periodiku
Zařazení RIV	J - Článek v odborném periodiku
Poddruh J	Článek ve WOS
Název	A NOVEL APPROACH FOR THE DETERMINATION OF MEMBRANE PROTEIN EXPRESSION LEVELS BASED ON LC-MS/MS
Tvůrce(i)	Hynek, R. (CZ) Maršálová, L. (CZ) Kučková, Š. (CZ) Koník, P. (CZ) Šantrůček, J. (CZ) Martinec, Jan (UEB-Q)_{RID, ORCID} Novotná, Z. (CZ) Kodíček, M. (CZ)
Zdroj.dok.	Journal of Liquid Chromatography & Related Technologies. - : Taylor & Francis - ISSN 1082-6076 Roč. 34, č. 12 (2011), s. 1004-1013
Poč.str.	10 s.
Jazyk dok.	eng - angličtina
Země vyd.	US - Spojené státy americké
Klíč. slova	expression levels ; isoxaben ; iTRAQ
Vědní obor RIV	CE - Biochemie
CEP	GA203/09/0036 GA ČR - Grantová agentura ČR
	LC06034 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy
CEZ	AV0Z50380511 - UEB-Q (2005-2011)
UT WOS	000291807100005
DOI	10.1080/10826076.2011.569808
Anotace	Membrane proteins play crucial roles in a number of cellular processes. Thus, the determination of their expression levels under various physiological conditions is crucial in many biological and biochemical studies. However, the extremely hydrophobic nature of proteins possessing transmembrane domains complicates their analysis. Therefore, we developed a new approach toward quantification of membrane proteins in complex mixtures combining reversed-phase chromatography on C4 resin and mass spectrometric techniques using iTRAQ labels. The method was tested on plasma membrane proteins obtained from cell cultures of A.t. by 2-phase partitioning of microsomal fractions. Then, the technique was applied for determination of the influence of the pesticide isoxaben on plasma membrane protein expression levels, particularly plasma membrane Hþ-ATPase. The developed technique seems to be promising for the determination of expression levels of membrane proteins in general.
Pracoviště	Ústav experimentální botaniky
Kontakt	David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469
Rok sběru	2012

Počet záznamů: 1