Počet záznamů: 1
Fatty acid modification of Wnt1 and Wnt3a at serine is prerequisite for lipidation at cysteine and is essential for Wnt signalling
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SYSNO ASEP 0364988 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Fatty acid modification of Wnt1 and Wnt3a at serine is prerequisite for lipidation at cysteine and is essential for Wnt signalling Tvůrce(i) Doubravská, Lenka (UMG-J)
Krausová, Michaela (UMG-J) RID
Gradl, D. (DE)
Vojtěchová, Martina (UMG-J) RID
Tůmová, Lucie (UMG-J)
Lukáš, Jan (UMG-J)
Valenta, Tomáš (UMG-J)
Pospíchalová, Vendula (UMG-J)
Fafílek, Bohumil (UMG-J) RID
Plachý, Jiří (UMG-J) RID
Sebesta, O. (CZ)
Kořínek, Vladimír (UMG-J) RIDZdroj.dok. Cellular Signalling. - : Elsevier - ISSN 0898-6568
Roč. 23, č. 5 (2011), s. 837-848Poč.str. 12 s. Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova Wnt signaling ; post-translational modification ; acylation Vědní obor RIV EB - Genetika a molekulární biologie CEP GA204/07/1567 GA ČR - Grantová agentura ČR 1M0506 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy CEZ AV0Z50520514 - UMG-J (2005-2011) UT WOS 000288977400011 DOI 10.1016/j.cellsig.2011.01.007 Anotace Mammalian Wnt ligands contain two types of post-translational modification: the covalent attachment of fatty acids at two distinct positions, and the N-glycosylation of multiple asparagines. We examined how these modifications contribute to the secretion, extracellular movement and signaling activity of mouse Wnt1 and Wnt3a ligands. We revealed that O-linked acylation of serine is required for the subsequent S-palmitoylation of cysteine. Interestingly, although double-acylation of Wnt1 was indispensable for signaling in mammalian cells, in Xenopus embryos the S-palmitoyl-deficient form retained the signaling activity. In the case of Wnt3a, the functional duality of the attached acyls was less prominent, since the ligand lacking S-linked palmitate was still capable of signaling in various cellular contexts. Finally, we show that the signaling competency of both Wnt1 and Wnt3a is related to their ability to associate with the extracellular matrix. Pracoviště Ústav molekulární genetiky Kontakt Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217 Rok sběru 2012
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