Počet záznamů: 1

Experimental Examination of EFL and MATX Eukaryotic Horizontal Gene Transfers: Coexistence of Mutually Exclusive Transcripts Predates Functional Rescue

SYSNO ASEP	0364810
Druh ASEP	J - Článek v odborném periodiku
Zařazení RIV	J - Článek v odborném periodiku
Poddruh J	Článek ve WOS
Název	Experimental Examination of EFL and MATX Eukaryotic Horizontal Gene Transfers: Coexistence of Mutually Exclusive Transcripts Predates Functional Rescue
Tvůrce(i)	Szabová, J. (CZ) Růžička, Petr (BC-A) Verner, Zdeněk (BC-A) Hampl, V. (CZ) Lukeš, Julius (BC-A)_{RID, ORCID}
Zdroj.dok.	Molecular Biology and Evolution. - : Oxford University Press - ISSN 0737-4038 Roč. 28, č. 8 (2011), s. 2371-2378
Poč.str.	8 s.
Jazyk dok.	eng - angličtina
Země vyd.	US - Spojené státy americké
Klíč. slova	EFL ; MATX ; horizontal gene transfer ; functional rescue ; RNAi ; Trypanosoma
Vědní obor RIV	EB - Genetika a molekulární biologie
CEP	GA204/09/1667 GA ČR - Grantová agentura ČR
	2B06129 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy
CEZ	AV0Z60220518 - PAU-O, BC-A (2005-2011)
UT WOS	000293304700018
DOI	10.1093/molbev/msr060
Anotace	Elongation factor 1 alpha (EF-1 alpha) and methionine adenosyl transferase (MAT) are enzymes with complicated evolutionary histories. These essential enzymes occur as two relatively divergent paralogs (EF-1 alpha/EFL, MAT/MATX) that have patchy distributions in eukaryotic lineages. To understand the evolution of these paralogs, we have performed in vivo experiments in Trypanosoma brucei – we have demonstrated that EF-1 alpha and MAT can be simultaneously expressed with EFL and MATX, respectively, without affecting the growth of the flagellates. After the endogenous MAT or EF-1 alpha was downregulated by RNA interference, MATX immediately substituted for its paralog, whereas EFL was not able to substitute for EF-1 alpha, leading to mortality. We conclude that MATX is naturally capable of evolving patchy paralog distribution via HGTs and/or long- term coexpression and differential losses. The capability of EFL to spread by HGT is lower.
Pracoviště	Biologické centrum (od r. 2006)
Kontakt	Dana Hypšová, eje@eje.cz, Tel.: 387 775 214
Rok sběru	2012

Počet záznamů: 1