Počet záznamů: 1

BAC Libraries from Wheat Chromosome 7D: Efficient Tool for Positional Cloning of Aphid Resistance Genes

SYSNO ASEP	0364122
Druh ASEP	J - Článek v odborném periodiku
Zařazení RIV	J - Článek v odborném periodiku
Poddruh J	Článek ve WOS
Název	BAC Libraries from Wheat Chromosome 7D: Efficient Tool for Positional Cloning of Aphid Resistance Genes
Tvůrce(i)	Šimková, Hana (UEB-Q)_{RID, ORCID} Šafář, Jan (UEB-Q)_{RID, ORCID} Kubaláková, Marie (UEB-Q)_RID Suchánková, Pavla (UEB-Q)_RID Čihalíková, Jarmila (UEB-Q)_RID Robert-Quatre, Heda (UEB-Q) Azhaguvel, P. (US) Weng, Y. Q. (US) Peng, J. (US) Lapitan, N. L. V. (US) Ma, Y. Q. (US) You, F. M. (US) Luo, M. C. (US) Bartoš, Jan (UEB-Q)_{RID, ORCID} Doležel, Jaroslav (UEB-Q)_{RID, ORCID}
Zdroj.dok.	Journal of Biomedicine and Biotechnology - ISSN 1110-7243 -, č. 302543 (2011), s. 1-11
Poč.str.	11 s.
Jazyk dok.	eng - angličtina
Země vyd.	US - Spojené státy americké
Klíč. slova	TRITICUM-AESTIVUM L. ; HEXAPLOID WHEAT ; BREAD WHEAT
Vědní obor RIV	EB - Genetika a molekulární biologie
CEP	GA521/07/1573 GA ČR - Grantová agentura ČR
	LC06004 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy
CEZ	AV0Z50380511 - UEB-Q (2005-2011)
UT WOS	000286232600001
DOI	10.1155/2011/302543
Anotace	Positional cloning in bread wheat is a tedious task due to its huge genome size and hexaploid character. BAC libraries represent an essential tool for positional cloning. However, wheat BAC libraries comprise more than milion clones, which makes thein screening very laborious. Here, we present a targeted approach based on chromosome-specific BAC libraries. Such libraries were constructed from flow-sorted arms of wheat chromosome 7D. A library from the short arm (7DS) consisting of 49,152 clones with 113 kb insert size represented 12.1 arm equivalents whereas a library from the long arm (7DL) comprised 50,304 clones of 116 kb providing 14.9x arm coverage. The 7DS library was PCR screened with markers linked to Russian wheat aphid resistance gene DnCI2401, the 7DL library was screened by hybridization with a probe linked to greenbug resistence gene Gb3. The small number of clones combined with high coverage made the screening highly efficient and cost effective.
Pracoviště	Ústav experimentální botaniky
Kontakt	David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469
Rok sběru	2012

Počet záznamů: 1