Počet záznamů: 1
The use of oligoperoxide-coated magnetic nanoparticles to label stem cells
- 1.
SYSNO ASEP 0363241 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název The use of oligoperoxide-coated magnetic nanoparticles to label stem cells Tvůrce(i) Šponarová, Daniela (UMCH-V)
Horák, Daniel (UMCH-V) RID, ORCID
Trchová, Miroslava (UMCH-V) RID, ORCID
Jendelová, Pavla (UEM-P) RID, ORCID
Herynek, V. (CZ)
Mitina, N. (UA)
Zaichenko, A. (UA)
Stoika, R. (UA)
Lesný, Petr (UEM-P)
Syková, Eva (UEM-P) RIDZdroj.dok. Journal of Biomedical Nanotechnology. - : American Scientific Publishers - ISSN 1550-7033
Roč. 7, č. 3 (2011), s. 384-394Poč.str. 11 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova magnetic ; nanoparticles ; stem cells Vědní obor RIV FH - Neurologie, neurochirurgie, neurovědy CEP GA203/09/1242 GA ČR - Grantová agentura ČR GAP503/10/0664 GA ČR - Grantová agentura ČR 1M0538 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy KAN201110651 GA AV ČR - Akademie věd KAN401220801 GA AV ČR - Akademie věd CEZ AV0Z40500505 - UMCH-V (2005-2011) AV0Z50390703 - UEM-P (2007-2013) UT WOS 000292858900008 DOI 10.1166/jbn.2011.1289 Anotace Iron oxide nanoparticles obtained by the coprecipitation of Fe(II) and Fe(III) salts and oxidation were coated with a novel poly(vinyl acetate-co-5-tert-(butylperoxy)-5-methylhex-1-en-3-yne-co-butyl acrylate-co-maleic anhydride) (PVBM) oligomer to ensure colloidal stability. The magnetic nanoparticles were thoroughly characterized by a range of physico-chemical methods, which proved the presence of the coating on the particles. Experiments with rat mesenchymal stem cells (rMSCs) confirmed that PVBM-coated γ-Fe2O3 nanoparticles were not cytotoxic and that the average efficiency of stem cell labeling was good and comparable to that obtained with commercial agents. The cells labeled with PVBM-coated γ-Fe2O3 nanoparticles displayed excellent contrast on magnetic resonance (MR) images. Such particles are thus promising for in vivo MR imaging of transplanted cells. Moreover, PVBM offers possibility of additional modification by grafting compounds that reduce non-specific protein adsorption. Pracoviště Ústav makromolekulární chemie Kontakt Eva Čechová, cechova@imc.cas.cz ; Tel.: 296 809 358 Rok sběru 2012
Počet záznamů: 1