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Poly(HEMA) brushes emerging as a new platform for direct detection of food pathogen in milk samples
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SYSNO ASEP 0362960 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Poly(HEMA) brushes emerging as a new platform for direct detection of food pathogen in milk samples Tvůrce(i) Rodriguez-Emmenegger, Cesar Adolfo (UMCH-V) RID
Avramenko, Oxana (UMCH-V)
Brynda, Eduard (UMCH-V) RID
Škvor, J. (CZ)
Bologna Alles, A. (UY)Zdroj.dok. Biosensors and Bioelectronics. - : Elsevier - ISSN 0956-5663
Roč. 26, č. 11 (2011), s. 4545-4551Poč.str. 7 s. Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova surface plasmon resonance ; food-borne pathogens ; polymer brushes Vědní obor RIV JA - Elektronika a optoelektronika, elektrotechnika CEP KAN200670701 GA AV ČR - Akademie věd GAP503/10/0664 GA ČR - Grantová agentura ČR CEZ AV0Z40500505 - UMCH-V (2005-2011) UT WOS 000293104100046 DOI 10.1016/j.bios.2011.05.021 Anotace The attachment of antibodies onto poly(HEMA) brushes prepared by surface initiated ATRP provided a successful way to fabricate SPR biosensors capable of in-real time detection Cronobacter at concentrations down to 106 cells mL−1 in milk. Non-specific responses to deposition of non-targeted compounds ruled out other biosensors based on poly(MeOEGMA) and poly(HOEGMA) brushes or SAMs of EG2/COEG and EG6 from detection of Cronobacter at low concentrations even if the lowest detection limit of 104 cells mL−1 in PBS was reached by biosensor based on SAM of EG6. Owing to well-controlled character of ATRP it was possible to minimize the thickness of the polymer brushes films so that the fouling could be still suppressed while the capture of antigens by antibodies immobilized on the brush layer could take place close to the gold SPR surface to provide a stronger optical response. Pracoviště Ústav makromolekulární chemie Kontakt Eva Čechová, cechova@imc.cas.cz ; Tel.: 296 809 358 Rok sběru 2012
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