Počet záznamů: 1

Hyperprolinemic larvae of the drosophilid fly, Chymomyza costata, survive cryopreservation in liquid nitrogen

SYSNO ASEP	0362689
Druh ASEP	J - Článek v odborném periodiku
Zařazení RIV	J - Článek v odborném periodiku
Poddruh J	Článek ve WOS
Název	Hyperprolinemic larvae of the drosophilid fly, Chymomyza costata, survive cryopreservation in liquid nitrogen
Tvůrce(i)	Košťál, Vladimír (BC-A)_{RID, ORCID} Zahradníčková, Helena (BC-A)_{RID, ORCID} Šimek, Petr (BC-A)_{RID, ORCID}
Celkový počet autorů	3
Zdroj.dok.	Proceedings of the National Academy of Sciences of the United States of America. - : National Academy of Sciences - ISSN 0027-8424 Roč. 108, č. 32 (2011), s. 13041-13046
Poč.str.	6 s.
Jazyk dok.	eng - angličtina
Země vyd.	US - Spojené státy americké
Klíč. slova	insect freeze tolerance ; cold hardiness ; glass transition
Vědní obor RIV	ED - Fyziologie
CEP	GA206/07/0269 GA ČR - Grantová agentura ČR
	GA203/09/2014 GA ČR - Grantová agentura ČR
CEZ	AV0Z50070508 - ENTU-I, BC-A (2005-2011)
UT WOS	000293691400023
DOI	10.1073/pnas.1107060108
Anotace	The larva of the drosophilid fly, Chymomyza costata, is probably the most complex metazoan organism that can survive submergence in liquid nitrogen (-196°C) in a fully hydrated state. We examined the associations between the physiological and biochemical parameters of differently acclimated larvae and their freeze tolerance. Profiling of 61 different metabolites identified proline as a prominent compound whose concentration increased from 20 to 147 mM during diapause transition and subsequent cold acclimation. This study provides direct evidence for the essential role of proline in high freeze tolerance. Differential scanning calorimetry analysis suggested that high proline levels, in combination with a relatively low content of osmotically active water and freeze dehydration, increased the propensity of the remaining unfrozen water to undergo a glass-like transition (vitrification) and thus facilitated the prevention of cryoinjury.
Pracoviště	Biologické centrum (od r. 2006)
Kontakt	Dana Hypšová, eje@eje.cz, Tel.: 387 775 214
Rok sběru	2012

Počet záznamů: 1