Počet záznamů: 1

Cleavage of Functionalized DNA Containing 5-Modified Pyrimidines by Type II Restriction Endonucleases

SYSNO ASEP	0360993
Druh ASEP	J - Článek v odborném periodiku
Zařazení RIV	J - Článek v odborném periodiku
Poddruh J	Článek ve WOS
Název	Cleavage of Functionalized DNA Containing 5-Modified Pyrimidines by Type II Restriction Endonucleases
Tvůrce(i)	Macíčková-Cahová, Hana (UOCHB-X)_{RID, ORCID} Pohl, Radek (UOCHB-X)_{RID, ORCID} Hocek, Michal (UOCHB-X)_{RID, ORCID}
Celkový počet autorů	3
Zdroj.dok.	Chembiochem. - : Wiley - ISSN 1439-4227 Roč. 12, č. 3 (2011), s. 431-438
Poč.str.	8 s.
Jazyk dok.	eng - angličtina
Země vyd.	DE - Německo
Klíč. slova	base-modified DNA ; DNA cleavage ; DNA polymerases ; nucleosides ; restriction endonucleases
Vědní obor RIV	CC - Organická chemie
CEP	LC512 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy
	GA203/09/0317 GA ČR - Grantová agentura ČR
CEZ	AV0Z40550506 - UOCHB-X (2005-2011)
UT WOS	000287718700016
DOI	10.1002/cbic.201000644
Anotace	A series of six pyrimidine modified dNTPs: 5-ethynyl- 5-phenyl- and 5-(3-nitrophenyl)-deoxycitidine and -deoxyuridine triphosphates were prepared and incorporated by primer extension using Vent (exo-) polymerase to specific DNA sequences within or next to the recognition sequences of selected restriction endonucleases. Then the cleavage of these pyrimidine-modified DNA sequences by 13 restriction enzymes was studied. While the presence of any modified C within the target sequence completely prevented any restriction cleavage, most enzymes tolerated the presence of 5-ethynylU and two of them even the presence of 5-phenyl- and 5-(3-nitrophenyl)U. Modifications outside the recognition sequence were tolerated apart from phenyl derivatives with PvuII enzyme. 5-EthynylC was used for protection of the recognition sequence from cleavage in presence of the second unmodified copy of the same sequence that was cleaved.
Pracoviště	Ústav organické chemie a biochemie
Kontakt	asep@uochb.cas.cz ; Kateřina Šperková, Tel.: 232 002 584 ; Viktorie Chládková, Tel.: 232 002 434
Rok sběru	2012

Počet záznamů: 1