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Sample preparation in separation of the extracellular chitinolytic enzymes of the human intestinal bacterium Clostridium paraputrificum J4 from the culture fluids
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SYSNO ASEP 0360819 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Sample preparation in separation of the extracellular chitinolytic enzymes of the human intestinal bacterium Clostridium paraputrificum J4 from the culture fluids Tvůrce(i) Tishchenko, Galina (UMCH-V) RID
Šimůnek, Jiří (UZFG-Y) RID
Bartoňová, Hana (UZFG-Y)
Dušková, Jarmila (UMCH-V) RID
Dohnálek, Jan (UMCH-V) RID
Ponomareva, Evgenia (UMCH-V)
Tennikova, T. (RU)Zdroj.dok. Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences. - : Elsevier - ISSN 1570-0232
Roč. 879, č. 22 (2011), s. 2175-2178Poč.str. 4 s. Jazyk dok. eng - angličtina Země vyd. NL - Nizozemsko Klíč. slova sample preparation ; culture fluids C. paraputrificum J4 ; chitinases Vědní obor RIV EE - Mikrobiologie, virologie CEP GA310/09/1407 GA ČR - Grantová agentura ČR GA525/05/2584 GA ČR - Grantová agentura ČR CEZ AV0Z40500505 - UMCH-V (2005-2011) AV0Z50450515 - UZFG-Y (2005-2011) UT WOS 000293262500023 DOI 10.1016/j.jchromb.2011.05.043 Anotace Membrane ultrafiltration (UF) was used in sample preparation of the culture fluids of the human intestinal bacterium Clostridium paraputrificum strain J4 containing seven extracellular chitinolytic isoenzymes (38–90 kDa). The subsequent filtration of the bacteria-free supernatants was carried out through Millipore membranes with cut-off 100 and 30 kDa for separation of undigested components of the culture medium and bacterial metabolites with molecular weight higher and lower than that of the target enzymes. On the basis of the obtained experimental results, the sample preparation procedure of the culture fluids of C. paraputrificum J4 was established to be used further in chromatographic separations of the chitinolytic enzymes. Pracoviště Ústav makromolekulární chemie Kontakt Eva Čechová, cechova@imc.cas.cz ; Tel.: 296 809 358 Rok sběru 2012
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