Počet záznamů: 1
Electrophoretic and zymographic characterization of proteins isolated by various extraction methods from ejaculated and capacitated boar sperms
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SYSNO ASEP 0360805 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Electrophoretic and zymographic characterization of proteins isolated by various extraction methods from ejaculated and capacitated boar sperms Tvůrce(i) Zigo, Michal (BTO-N) RID
Jonáková, Věra (BTO-N) RID
Maňásková-Postlerová, Pavla (BTO-N) ORCIDCelkový počet autorů 3 Zdroj.dok. Electrophoresis. - : Wiley - ISSN 0173-0835
Roč. 32, č. 11 (2011), s. 1309-1318Poč.str. 10 s. Jazyk dok. eng - angličtina Země vyd. DE - Německo Klíč. slova Extraction methods ; Sperm proteins ; Substrate zymography Vědní obor RIV CE - Biochemie CEP GA303/09/1285 GA ČR - Grantová agentura ČR NS10009 GA MZd - Ministerstvo zdravotnictví 1M06011 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy CEZ AV0Z50520701 - BTO-N (2007-2013) UT WOS 000291555900007 DOI 10.1002/elps.201000558 Anotace Recognition and the binding of spermatozoa to the zona pellucida (ZP) is a crucial step in the fertilization process. Despite longstanding research efforts to identify the sperm proteins that recognize ZP receptors, their precise determination still remains questionable in various mammalian species. Sperm proteins of ejaculated and in vitro capacitated boar sperms were isolated by agents as follows: Triton X-100, Triton X-114, acetic acid, SDS, n-octyl-β-D-glucopyranosid, extraction by freezing-thawing and characterized by 1D, 2D electrophoreses glycoprotein staining and substrate zymographic methods. Results have shown the quantitative and qualitative differences in 1D, 2D protein and glycoprotein profiles depending upon the isolation protocol. Differences were observed between protein profiles of ejaculated and capacitated sperms as well. Zymograms supported the prediction that using various isolation protocols gives various profiles of enzymatically active molecules. Pracoviště Biotechnologický ústav Kontakt Monika Kopřivová, Monika.Koprivova@ibt.cas.cz, Tel.: 325 873 700 Rok sběru 2012
Počet záznamů: 1