Počet záznamů: 1
Biosynthesis of omega-alicyclic fatty acids induced by cyclic precursors and change of membrane fluidity in thermophilic bacteria Geobacillus stearothermophilus and Meiothermus ruber
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SYSNO ASEP 0360791 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Biosynthesis of omega-alicyclic fatty acids induced by cyclic precursors and change of membrane fluidity in thermophilic bacteria Geobacillus stearothermophilus and Meiothermus ruber Tvůrce(i) Siřišťová, L. (CZ)
Luhový, R. (CZ)
Sigler, Karel (MBU-M) RID
Řezanka, Tomáš (MBU-M) ORCIDZdroj.dok. Extremophiles. - : Springer - ISSN 1431-0651
Roč. 15, č. 3 (2011), 423-429Poč.str. 7 s. Jazyk dok. eng - angličtina Země vyd. JP - Japonsko Klíč. slova Thermophilic bacteria ; Geobacillus ; Meiothermus Vědní obor RIV EE - Mikrobiologie, virologie CEZ AV0Z50200510 - MBU-M (2005-2011) UT WOS 000290037700012 DOI 10.1007/s00792-011-0373-4 Anotace Two thermophilic strains belonging to Geobacillus stearothermophilus and Meiothermus ruber, which naturally do not synthesize omega-alicyclic fatty acids (omega-FAs) were cultivated with cyclopropyl, cyclobutyl, cyclopentyl and cyclohexyl carboxylic acids. Gas chromatography-mass spectrometry analysis of fatty acid methyl and picolinyl esters showed that both strains are able to synthesize omega-FAs when cultivated with the appropriate precursor. The incorporation of cyclic acids influenced the whole FA composition as well as membrane fluidity. Membrane fluidity of intact cells was studied by measuring the fluorescence polarisation of the probe l,6-diphenyl-1,3,5-hexatriene incorporated into membrane lipid bilayers. Cytoplasmic membrane became more fluid with increasing content of omega-FAs. This is caused by considerable changes in lipid packing within the membrane induced by the presence of omega-FAs not found in the natural environment of Geobacillus and Meiothermus strains Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2012
Počet záznamů: 1