Počet záznamů: 1
Effect of epidermal growth factor-like peptides on pig cumulus cell expansion, oocyte maturation and acquisition of developmental competence in vitro: comparison with gonadotropins
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SYSNO ASEP 0359775 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Effect of epidermal growth factor-like peptides on pig cumulus cell expansion, oocyte maturation and acquisition of developmental competence in vitro: comparison with gonadotropins Tvůrce(i) Procházka, Radek (UZFG-Y) RID, ORCID
Petlach, Michal (UZFG-Y)
Nagyová, Eva (UZFG-Y) RID, ORCID
Němcová, Lucie (UZFG-Y) RIDZdroj.dok. Reproduction. - : BioScientifica - ISSN 1470-1626
Roč. 141, č. 4 (2011), s. 425-435Poč.str. 11 s. Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova activatd protein kinase ; EGF like factor ; meiotic resumption Vědní obor RIV EB - Genetika a molekulární biologie CEP GA523/08/0111 GA ČR - Grantová agentura ČR QI101A166 GA MZe - Ministerstvo zemědělství CEZ AV0Z50450515 - UZFG-Y (2005-2011) UT WOS 000288500500005 DOI 10.1530/REP-10-0418 Anotace The aim of this work was to assess the FSH-stimulated expression of epidermal growth factor (EGF)-like peptides in cultured cumulus-oocyte complexes (COCs) and to find out the effect of the peptides on cumulus expansion, oocyte maturation and acquisition of developmental competence in vitro. FSH promptly stimulated expression of amphiregulin (AREG) and epiregulin (EREG), but notbetacellulin (BTC) in the cultured COCs. Both FSH and the EGF-like peptides significantly stimulated expression of expansion related genes (PTGS2, TNFAIP6 and HAS2) in the COCs and stimulated maturation of oocytes and expansion of cumulus cells. In contrast to FSH, AREG and EREG neither stimulated expression of CYP11A1 in the COCs nor an increase in progesterone production by cumulus cells. Significantly more oocytes stimulated with AREG and/or EREG developed to blastocyst stage after parthenogenetic activation when compared to oocytes stimulated with FSH alone or combinations of FSH/LH or PMSG/hCG. Pracoviště Ústav živočišné fyziologie a genetiky Kontakt Jana Zásmětová, knihovna@iapg.cas.cz, Tel.: 315 639 554 Rok sběru 2012
Počet záznamů: 1